Islet 1 Recombinant antibody, PBS Only

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  WB analysis using 83784-4-RR (same clone as 83784-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83784-4-RR (Islet 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83784-4-RR (same clone as 83784-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83784-4-RR (Islet 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83784-4-RR (same clone as 83784-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83784-4-RR (Islet 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 83784-4-RR (same clone as 83784-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Islet 1 antibody (83784-4-RR, Clone: 240833D7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83784-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83784-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83784-4-RR against Human Islet 1 were performed. The affinity constant is below 1 pM.

Islet 1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 83784-4-PBS

Clone No.240833D7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

ISL1, 240833D7, Insulin gene enhancer protein ISL-1, Isl 1, ISL LIM homeobox 1

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Product Information

83784-4-PBS targets Islet 1 in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Islet 1 fusion protein Ag33541
• Full Name: ISL LIM homeobox 1
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39-45 kDa
• GenBank Accession Number: BC031213
• Gene Symbol: Islet 1
• Gene ID (NCBI): 3670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61371
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ISL1 belongs to the LIM homeodomain transcription factor family that binds to the enhancer region of the INS gene. It plays a pivotal role in cardiogenesis, and lacking ISL1 expression results in growth arrest or displays profound defects in heart development, including atria, ventricle, and the inflow and outflow tracts. Ubiquitinated probably by WWP1 E3 ubiquitin ligase; ubiquitination is followed by protein degradation.