JAK3 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 80331-1-RR (same clone as 80331-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80331-1-RR (JAK3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80331-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 80331-1-RR (same clone as 80331-1-PBS)

  IP result of anti-JAK3 (IP:80331-1-RR, 4ug; Detection:80331-1-RR 1:1000) with HEK-293 cells lysate 1640 ug. This data was developed using the same antibody clone with 80331-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 80331-1-RR (same clone as 80331-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 80331-1-RR (JAK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80331-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 80331-1-RR (same clone as 80331-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 80331-1-RR (JAK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80331-1-PBS in a different storage buffer formulation.

JAK3 Uni-rAb^® Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 80331-1-PBS

Clone No.3N1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

3N1, EC:2.7.10.2, JAK 3, JAK-3, JAKL

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Product Information

80331-1-PBS targets JAK3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Janus kinase 3 (a protein tyrosine kinase, leukocyte)
• Calculated Molecular Weight: 1124 aa, 125 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: BC028068
• Gene Symbol: JAK3
• Gene ID (NCBI): 3718
• RRID: AB_2918888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P52333
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

JAK3 is a non-receptor tyrosine kinase, predominantly expressed in hematopoietic cells and that has been implicated in the signal transduction of the common gamma chain subfamily of cytokine receptors. As a result, JAK3 plays an essential role in hematopoieisis during T cell development. JAK3 belongs to the Janus kinase family of receptor-associated tyrosine kinases located in cytoplasm adjacent to the plasma membrane. (PMID: 19747563, PMID: 33767339)