JAKMIP1 Recombinant monoclonal antibody

• 

  WB analysis using 86257-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86257-3-RR (JAKMIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of Jurkat using 86257-3-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using JAKMIP1 antibody (86257-3-RR, Clone: 251026C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HEK-293 using 86257-3-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using JAKMIP1 antibody (86257-3-RR, Clone: 251026C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 86257-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86257-3-RR against Human JAKMIP1 were performed. The affinity constant is below 1 pM.

JAKMIP1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86257-3-RR

Clone No.251026C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

GABA-B receptor-binding protein, GABABRBP, JAMIP1, janus kinase and microtubule interacting protein 1, Janus kinase and microtubule-interacting protein 1

---

Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells
• Positive IF/ICC detected in: HEK-293 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86257-3-RR targets JAKMIP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag4816

  Product name: Recombinant human JAKMIP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 277-626 aa of BC047075

  Sequence: VQDQHMDERDVRRFQLKIAELNSVIRKLEDRNTLLADERNELLKRSRETEVQLKPLVEKNKRMNKKNEDLLQSIQRMEEKIKNLTRENVEMKEKLSAQASLKRHTSLNDLSLTRDEQEIEFLRLQVLEQQHVIDDLSLERERLLRSKRHRGKSLKPPKKHVVETFFGFDEESVDSETLSETSYNTDRTDRTPATPEEDLDDATAREEADLRFCQLTREYQALQRAYALLQEQVGGTLDAEREARTREQLQADLLRCQAKIEDLEKLLVEKGQDSKWVEEKQLLIRTNQDLLEKIYRLEMEENQLKNEMQDAKDQNELLEFRVLELEVRDSICCKLSNGADILFEPKLKFM

• Full Name: janus kinase and microtubule interacting protein 1
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 73-75 kDa
• GenBank Accession Number: BC047075
• Gene Symbol: JAKMIP1
• Gene ID (NCBI): 152789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96N16
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.