JIP1/IB-1 Polyclonal antibody

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  WB analysis of fetal human brain using 14568-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 14568-1-AP (JIP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14568-1-AP

  IP result of anti-JIP1/IB-1 (IP:14568-1-AP, 4ug; Detection:14568-1-AP 1:500) with mouse brain tissue lysate 1840 ug.

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  IP experiment of rat brain using 14568-1-AP

  IP result of anti-JIP1/IB-1 (IP:14568-1-AP, 4ug; Detection:14568-1-AP 1:500) with rat brain tissue lysate 1120 ug.

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  IHC staining of human oesophagus cancer using 14568-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 14568-1-AP (JIP1/IB-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 14568-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 14568-1-AP (JIP1/IB-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14568-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14568-1-AP (JIP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 14568-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14568-1-AP (JIP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 14568-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14568-1-AP (JIP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 14568-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14568-1-AP (JIP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human oesophagus cancer using 14568-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 14568-1-AP (JIP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus cancer using 14568-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 14568-1-AP (JIP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of PC-12 using 14568-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using JIP1/IB-1 antibody (14568-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

JIP1/IB-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14568-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

MAPK8IP1, Islet-brain 1, Islet brain 1, IB-1, IB1

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Tested Applications

• Positive WB detected in: fetal human brain tissue
• Positive IP detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human oesophagus cancer tissue, mouse brain tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

14568-1-AP targets JIP1/IB-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: JIP1/IB-1 fusion protein Ag6150
• Full Name: mitogen-activated protein kinase 8 interacting protein 1
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 97-116 kDa
• GenBank Accession Number: BC068470
• Gene Symbol: MAPK8IP1/IB-1
• Gene ID (NCBI): 9479
• RRID: AB_2281682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UQF2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Curr Biol; Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axons; Authors - Sara C Sousa
mouse	IF	ACS Chem Neurosci; Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.; Authors - Lei Wu
human	IP, WB	Front Physiol; Ras-Related C3 Botulinum Toxin Substrate 1 Combining With the Mixed Lineage Kinase 3- Mitogen-Activated Protein Kinase 7- c-Jun N-Terminal Kinase Signaling Module Accelerates Diabetic Nephropathy.; Authors - Changjiang Ying
mouse	WB	Saudi Pharm J; iTRAQ-derived quantitative proteomics uncovers the neuroprotective property of bexarotene in a mice model of cerebral ischemia-reperfusion injury.; Authors - Hailin Liu
mouse	WB	Cell Rep; SFRS11 Loss Leads to Aging-Associated Cognitive Decline by Modulating LRP8 and ApoE.; Authors - Obayed Raihan
human	WB	Oncotarget; Autophagy-related gene expression is an independent prognostic indicator of glioma.; Authors - Huixue Zhang