CoraLite® Plus 488-conjugated JNK Recombinant antibody

JNK Recombinant Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6J16

Cat no : CL488-81629

Synonyms

c Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1

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Validation Data Gallery

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  IF Staining of Jurkat using CL488-81629

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 JNK antibody (CL488-81629, Clone: 6J16 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-81629

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human JNK (CL488-81629, Clone:6J16) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF detected in: Jurkat cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-81629 targets JNK in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: JNK fusion protein Ag1639
• Full Name: mitogen-activated protein kinase 8
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 44-48 kDa, 50-55 kDa
• GenBank Accession Number: BC130572
• Gene Symbol: MAPK8
• Gene ID (NCBI): 5599
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has some isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot(PMID:11062067 ). This antibody can recognize JNK1, JNK2 and JNK3.