|Positive WB detected in||mouse heart tissue, human heart tissue, HeLa cells, T-47D cells, A431 cells|
|Positive IHC detected in||human pancreas cancer tissue, human breast cancer tissue, human colon cancer tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11146-1-AP targets Gamma Catenin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Gamma Catenin fusion protein Ag1627|
|Full Name||junction plakoglobin|
|Calculated molecular weight||82 kDa|
|Observed molecular weight||82 kDa|
|GenBank accession number||BC011865|
|Gene ID (NCBI)||3728|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.
Biochem Biophys Res Commun
Plakoglobin is involved in cytoskeletal rearrangement of podocytes under the regulation of UCH-L1.
Quantitative Proteomic Profiling the Molecular Signatures of Annexin A5 in Lung Squamous Carcinoma Cells.
Loss of giant obscurins from breast epithelium promotes epithelial-to-mesenchymal transition, tumorigenicity and metastasis.
J Clin Invest
The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.