FcZero-rAb® Biotin Anti-Human Jagged1/CD339 Rabbit Recombinant Antibody

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• FcZero-rAb® Biotin Anti-Human Jagged1/CD339 Rabbit Recombinant Antibody (Biotin-FcA98243)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

Jagged1/CD339 FcZero-rAb^® Recombinant Antibody for FC, ELISA

Cat No. Biotin-FcA98243

Clone No.241412A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, ELISA

Synonyms

JAG1, Jagged1, CD339, HJ1, Protein jagged-1

• 

  FC experiment of Transfected HEK-293T using Biotin-FcA98243

  1x10^6 HEK-293T cells or Jagged1/CD339 transfected HEK-293T cells were surface stained with 0.25 ug Biotin Anti-Human Jagged1/CD339 Rabbit RecAb (Biotin-FcA98243, Clone: 241412A6) and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of Transfected HEK-293T using Biotin-FcA98243

  1x10^6 HEK-293T cells or Jagged1/CD339 transfected HEK-293T cells were surface stained with 0.25 ug Biotin Anti-Human Jagged1/CD339 Rabbit RecAb (Biotin-FcA98243, Clone: 241412A6) (red) or FcZero-rAb® Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: Transfected HEK-293T cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98243 targets Jagged1/CD339 in FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: jagged 1 (Alagille syndrome)
• Calculated Molecular Weight: 1218 aa, 134 kDa
• GenBank Accession Number: BC098393
• Gene Symbol: Jagged1
• Gene ID (NCBI): 182
• RRID: AB_3746993
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78504
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Jagged1 (JAG1), also known as CD339, is a type I, membrane-anchored, multi-domain protein and a ligand of the Notch receptor (PMID: 15358557). Jagged1 is composed of a relatively small intracellular domain, a transmembrane domain, and a larger extracellular component containing a cystine-rich region, 16 EGF-like repeats, a DSL domain, and a signal peptide (PMID: 15358557; 26548814). It is widely expressed throughout mammalian development, across many tissues and developmental stages (PMID: 26548814). The Jagged1-NOTCH interaction can trigger the Notch signaling and activate transcription factors that play key roles in cell differentiation and morphogenesis.