Tested Applications
| Positive IHC detected in | human colon tissue, human kidney tissue, mouse heart tissue, mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20546-1-AP targets KANK2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | KN motif and ankyrin repeat domains 2 |
| Calculated Molecular Weight | 91 kDa |
| GenBank Accession Number | NM_001136191 |
| Gene Symbol | KANK2 |
| Gene ID (NCBI) | 25959 |
| RRID | AB_10694286 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q63ZY3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KANK2, also named as ANKRD25, KIAA1518, MXRA3 and SIP, is found as a candidate tumor suppressor for renal cell carcinoma. It is an ankyrin-repeat domain-containing protein. KANK2 has an identical role in the formation of actin stress fibers. The antibody is specific to KANK2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KANK2 antibody 20546-1-AP | Download protocol |
| IHC protocol for KANK2 antibody 20546-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KANK2 Polyclonal antibody (Cat# 20546-1-AP) has 1 total citation. It appears in Laboratory Medicine (2024), where it was used for IHC to identify biomarkers associated with pathological tumor staging and their utility in the diagnosis and prognosis of prostate cancer. The research field is oncology, specifically prostate cancer biomarker discovery and prognostic evaluation.
| Species | Application | Title |
|---|---|---|
Lab Med Identification of biomarkers associated with pathological tumor staging and their utility in the diagnosis and prognosis of prostate cancer |
