Tested Applications
| Positive WB detected in | mouse heart tissue, mouse kidney tissue, Transfected HEK-293 cells, mouse lung tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21733-1-AP targets KANK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16416 Product name: Recombinant human KANK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-215 aa of BC098105 Sequence: MAQVLHVPAPFPGTPGPASPPAFPAKDPDPPYSVETPYGYRLDLDFLKYVDDIEKGHTLRRVAVQRRPRLSSLPRGPGSWWTSTESLCSNASGDSRHSAYSYCGRGFYPQYGALETRGGFNPRVERTLLDARRRLEDQAATPTGLGSLTPSAAGSTASLVGVGLPPPTPRSSGLSTPVPPSAGHLAHVREQMAGALRKLRQLEEQVKLIPVLQVK Predict reactive species |
| Full Name | KN motif and ankyrin repeat domains 2 |
| Calculated Molecular Weight | 851 aa, 91 kDa |
| Observed Molecular Weight | 95-100 kDa |
| GenBank Accession Number | BC098105 |
| Gene Symbol | KANK2 |
| Gene ID (NCBI) | 25959 |
| RRID | AB_10732728 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q63ZY3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Involved in transcription regulation by sequestering nuclear receptor coactivators, such as NCOA1, NCOA2 and NCOA3, in the cytoplasm; the function is deregulated by phosphorylation. May be involved in the control of cytoskeleton formation by regulating actin polymerization. Involved in regulation of caspase-independent apoptosis; proposed to sequester AIFM1 in mitochondria and apoptotic stimuli lead to its proteasomal degradation allowing the release of AIFM1 to the nucleus. May be involved in promotion of cell proliferation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KANK2 antibody 21733-1-AP | Download protocol |
| IHC protocol for KANK2 antibody 21733-1-AP | Download protocol |
| WB protocol for KANK2 antibody 21733-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KANK2 Polyclonal antibody (catalog number 21733-1-AP) has 6 total citations. These appear in Biofabrication, Biochemical Pharmacology, Journal of Cell Biology, American Journal of Physiology—Cell Physiology (two papers), and Journal of Biological Chemistry. Associated research fields include gut/microbiome interface studies, skin and scar therapy, RNA-binding protein translation, bladder contractility, podocyte FcRn signaling, and KANK2 mutation-related nephrotic syndrome. Applications cited include Western blot and immunofluorescence.
| Species | Application | Title |
|---|---|---|
Biofabrication An in vitro intestinal platform with a self-sustaining oxygen gradient to study the human gut/microbiome interface. | ||
Biochem Pharmacol From stem cells to skin: ADSCP6 peptide's role in transforming scar therapy | ||
J Cell Biol The RNA-binding protein Fus directs translation of localized mRNAs in APC-RNP granules. | ||
Am J Physiol Cell Physiol Itga8-Cre-mediated deletion of YAP and TAZ impairs bladder contractility with minimal inflammation and chondrogenic differentiation | ||
Am J Physiol Cell Physiol Knockout of the neonatal Fc Receptor (FcRn) in cultured podocytes alters IL-6 signaling and the actin cytoskeleton. | ||
J Biol Chem Nephrotic syndrome-associated mutation of KANK2 induces pathologic binding competition with physiological interactor KIF21A. |
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer reported a strong band at the expected molecular weight when using the antibody for Western Blot at a 1:1000 dilution. Overall, the feedback indicates reliable performance and clear specificity in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Bryce (Verified Customer) (09-12-2022) | Strong band at expected molecular weight.
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