KAP1 Recombinant antibody

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  WB analysis using 85693-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85693-4-RR (KAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85693-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KAP1 antibody (85693-4-RR, Clone: 243043D10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85693-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85693-4-RR against Human KAP1 were performed. The affinity constant is 8.82 nM.

KAP1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85693-4-RR

Clone No.243043D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

TRIM28, E3 SUMO-protein ligase TRIM28, EC:2.3.2.27, KAP 1, KAP-1

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, MCF-7 cells, PC-3 cells, OVCAR-3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85693-4-RR targets KAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KAP1 fusion protein Ag7350
• Full Name: tripartite motif-containing 28
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 100-120 kDa
• GenBank Accession Number: BC004978
• Gene Symbol: KAP1
• Gene ID (NCBI): 10155
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13263
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )