KATNAL1 Polyclonal antibody

• 

  WB analysis of HEK-293 using 20166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20166-1-AP (KATNAL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20166-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20166-1-AP (KATNAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 20166-1-AP

  IP result of anti-KATNAL1 (IP:20166-1-AP, 4ug; Detection:20166-1-AP 1:4000) with HeLa cells lysate 1280 ug.

KATNAL1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 20166-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

p60 katanin-like 1, p60 katanin like 1, Katanin p60 subunit A-like 1, katanin p60 subunit A like 1, Katanin p60 ATPase-containing subunit A-like 1

---

Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, HeLa cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20166-1-AP targets KATNAL1 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KATNAL1 fusion protein Ag13960
• Full Name: katanin p60 subunit A-like 1
• Calculated Molecular Weight: 490 aa, 55 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC000612
• Gene Symbol: KATNAL1
• Gene ID (NCBI): 84056
• RRID: AB_2878651
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BW62
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KATNAL1 belongs to a family of microtubule-severing proteins and contains an N-terminal microtubule interaction and trafficking (MIT) domain, an ATPase AAA-type core domain, and a C-terminal Vps4_C domain. KATNAL1 is involved in regulating microtubule dynamics, which is essential for spermiogenesis and male fertility. It severs microtubules in an ATP-dependent manner, promoting rapid reorganization of cellular microtubule arrays(PMID: 22654668). In neurons, KATNAL1 plays a role in microtubule rearrangement during neuronal migration and process elongation. Additionally, mutations in KATNAL1 have been associated with intellectual disability and microcephaly in humans(PMID: 30448058).