KBTBD10 Polyclonal antibody

KBTBD10 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25577-1-AP

Publications (1)

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

KBTBD10, Kel like protein 23, Kelch related protein 1, Kelch-like protein 41, KLH41, KLHL41, KRP1, SARCOSIN

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIHC

WB analysis using 25577-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25577-1-AP (KBTBD10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 25577-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25577-1-AP (KBTBD10 Antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skeletal muscle using 25577-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25577-1-AP (KBTBD10 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 25577-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25577-1-AP (KBTBD10 Antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human skeletal muscle using 25577-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25577-1-AP (KBTBD10 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for KBTBD10 in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected in	human heart tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25577-1-AP targets KBTBD10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag22311 Product name: Recombinant human KBTBD10 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 205-382 aa of BC006534 Sequence: RTDKENRVKNLSEVFDCIRFRLMTEKYFKDHVEKDDIIKSNPDLQKKIKVLKDAFAGKLPEPSKNAAKTGAGEVNGDVGDEDLLPGYLNDIPRHGMFVKDLILLVNDTAAVAYDPTENECYLTALAEQIPRNHSSIVTQQNQIYVVGGLYVDEENKDQPLQSYFFQLDSIASEWVGLP Predict reactive species
Full Name	kelch repeat and BTB (POZ) domain containing 10
Calculated Molecular Weight	606 aa, 68 kDa
Observed Molecular Weight	68 kDa
GenBank Accession Number	BC006534
Gene Symbol	KBTBD10
Gene ID (NCBI)	10324
RRID	AB_2880137
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O60662
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KBTBD10, also named as KBTBD10 or KRP1, is a 606 amino acid protein, which contains one BACK (BTB/Kelch associated) domain, one BTB (POZ) domain and five Kelch repeats. KBTBD10 localizes in the cytoplasm and is expressed in Sarcomeric muscle. KBTBD10 is involved in skeletal muscle development and differentiation. KBTBD10 regulates proliferation and differentiation of myoblasts and plays a role in myofibril assembly by promoting lateral fusion of adjacent thin fibrils into mature, wide myofibrils.

Protocols

Product Specific Protocols
IHC protocol for KBTBD10 antibody 25577-1-AP	Download protocol
WB protocol for KBTBD10 antibody 25577-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The KBTBD10 Polyclonal antibody (Cat# 25577-1-AP) has 1 total citation. The cited publication appeared in International Immunopharmacology (2026), investigating the ability of KBTBD10 to inhibit non-small cell lung cancer mediated by LINC01585. The associated research field is cancer biology, specifically focusing on the role of KBTBD10 in tumor suppression within the context of lung cancer and lncRNA-mediated regulation.