KBTBD10 Polyclonal antibody

KBTBD10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25577-1-AP

Synonyms

KBTBD10, Kel like protein 23, Kelch related protein 1, Kelch-like protein 41, KLH41, KLHL41, KRP1, SARCOSIN

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Validation Data Gallery

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  WB analysis using 25577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25577-1-AP (KBTBD10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 25577-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25577-1-AP (KBTBD10 Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skeletal muscle using 25577-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25577-1-AP (KBTBD10 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 25577-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25577-1-AP (KBTBD10 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 25577-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25577-1-AP (KBTBD10 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue
• Positive IHC detected in: human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25577-1-AP targets KBTBD10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KBTBD10 fusion protein Ag22311
• Full Name: kelch repeat and BTB (POZ) domain containing 10
• Calculated Molecular Weight: 606 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC006534
• Gene Symbol: KBTBD10
• Gene ID (NCBI): 10324
• RRID: AB_2880137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KBTBD10, also named as KBTBD10 or KRP1, is a 606 amino acid protein, which contains one BACK (BTB/Kelch associated) domain, one BTB (POZ) domain and five Kelch repeats. KBTBD10 localizes in the cytoplasm and is expressed in Sarcomeric muscle. KBTBD10 is involved in skeletal muscle development and differentiation. KBTBD10 regulates proliferation and differentiation of myoblasts and plays a role in myofibril assembly by promoting lateral fusion of adjacent thin fibrils into mature, wide myofibrils.