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- KD/KO Validated
Kv4.2 Polyclonal antibody
Kv4.2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 21298-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, DU 145 cells, HeLa cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 7 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
21298-1-AP targets Kv4.2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Kv4.2 fusion protein Ag15879 |
Full Name | potassium voltage-gated channel, Shal-related subfamily, member 2 |
Calculated Molecular Weight | 630 aa, 71 kDa |
Observed Molecular Weight | 70-80 kDa |
GenBank Accession Number | BC110449 |
Gene Symbol | KCND2 |
Gene ID (NCBI) | 3751 |
RRID | AB_10733102 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Voltage-gated potassium or Kv channels, specifically those mediating low threshold, rapidly inactivating Ito and IA currents, are known to regulate cardiac and neuronal membrane excitability, respectively (PMID: 12829703). Voltage-gated potassium channel subunit Kv4.2, encoded by the KCND2 gene, belongs to the potassium channel family and D (Shal) subfamily. It is a pore-forming alpha subunit of voltage-gated rapidly inactivating A-type potassium channels. Kv4.2 is highly expressed in the brain (PMID: 10551270). It is a major constituent of A-type potassium currents and a key regulator of neuronal membrane excitability (PMID: 22539834).
Protocols
Product Specific Protocols | |
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WB protocol for Kv4.2 antibody 21298-1-AP | Download protocol |
IHC protocol for Kv4.2 antibody 21298-1-AP | Download protocol |
IF protocol for Kv4.2 antibody 21298-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Neurobiol Dis MicroRNA inhibition upregulates hippocampal A-type potassium current and reduces seizure frequency in a mouse model of epilepsy. | ||
Sci Rep Mir324 knockout regulates the structure of dendritic spines and impairs hippocampal long-term potentiation | ||
eNeuro Estradiol- and Progesterone-Associated Changes in microRNA-Induced Silencing and Reduced Antiseizure Efficacy of an Antagomir in Female Mice | ||
JCI Insight Indoxyl sulfate reduces Ito,f by activating ROS/MAPK and NF-κB signaling pathways. | ||
Biochem Biophys Res Commun Pinocembrin alleviates the susceptibility to atrial fibrillation in isoproterenol-induced rats | ||
Res Sq Age-dependent regulation of dendritic spine density and protein expression in Mir324 KO mice |