Kv4.2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Kv4.2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 21298-1-AP

Synonyms

KCND2, Voltage-gated potassium channel subunit Kv4.2, RK5, Potassium voltage-gated channel subfamily D member 2, KIAA1044

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Validation Data Gallery

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  WB analysis of A549 using 21298-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21298-1-AP (Kv4.2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21298-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21298-1-AP (Kv4.2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 21298-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 21298-1-AP (Kv4.2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 21298-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21298-1-AP (Kv4.2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21298-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21298-1-AP (Kv4.2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 21298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 21298-1-AP (Kv4.2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 21298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 21298-1-AP (Kv4.2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 21298-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Kv4.2 antibody (21298-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, DU 145 cells, HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

21298-1-AP targets Kv4.2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Kv4.2 fusion protein Ag15879
• Full Name: potassium voltage-gated channel, Shal-related subfamily, member 2
• Calculated Molecular Weight: 630 aa, 71 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC110449
• Gene Symbol: KCND2
• Gene ID (NCBI): 3751
• RRID: AB_10733102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Voltage-gated potassium or Kv channels, specifically those mediating low threshold, rapidly inactivating Ito and IA currents, are known to regulate cardiac and neuronal membrane excitability, respectively (PMID: 12829703). Voltage-gated potassium channel subunit Kv4.2, encoded by the KCND2 gene, belongs to the potassium channel family and D (Shal) subfamily. It is a pore-forming alpha subunit of voltage-gated rapidly inactivating A-type potassium channels. Kv4.2 is highly expressed in the brain (PMID: 10551270). It is a major constituent of A-type potassium currents and a key regulator of neuronal membrane excitability (PMID: 22539834).

Publications

Species	Application	Title
mouse		Neurobiol Dis; MicroRNA inhibition upregulates hippocampal A-type potassium current and reduces seizure frequency in a mouse model of epilepsy.; Authors - Durgesh Tiwari
mouse	WB	Sci Rep; Mir324 knockout regulates the structure of dendritic spines and impairs hippocampal long-term potentiation; Authors - Emma V Parkins
mouse	WB	eNeuro; Estradiol- and Progesterone-Associated Changes in microRNA-Induced Silencing and Reduced Antiseizure Efficacy of an Antagomir in Female Mice; Authors - Durgesh Tiwari
rat	WB,IHC,IF	JCI Insight; Indoxyl sulfate reduces Ito,f by activating ROS/MAPK and NF-κB signaling pathways.; Authors - Jing Yang
rat	WB	Biochem Biophys Res Commun; Pinocembrin alleviates the susceptibility to atrial fibrillation in isoproterenol-induced rats; Authors - Zhangchi Liu
mouse	WB	Res Sq; Age-dependent regulation of dendritic spine density and protein expression in Mir324 KO mice; Authors - Emma V Parkins