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Kv4.2 Polyclonal antibody

Kv4.2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 21298-1-AP

Synonyms

KCND2, Voltage-gated potassium channel subunit Kv4.2, RK5, Potassium voltage-gated channel subfamily D member 2, KIAA1044



Tested Applications

Positive WB detected inA549 cells, DU 145 cells, HeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21298-1-AP targets Kv4.2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Kv4.2 fusion protein Ag15879
Full Name potassium voltage-gated channel, Shal-related subfamily, member 2
Calculated Molecular Weight 630 aa, 71 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC110449
Gene Symbol KCND2
Gene ID (NCBI) 3751
RRIDAB_10733102
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Voltage-gated potassium or Kv channels, specifically those mediating low threshold, rapidly inactivating Ito and IA currents, are known to regulate cardiac and neuronal membrane excitability, respectively (PMID: 12829703). Voltage-gated potassium channel subunit Kv4.2, encoded by the KCND2 gene, belongs to the potassium channel family and D (Shal) subfamily. It is a pore-forming alpha subunit of voltage-gated rapidly inactivating A-type potassium channels. Kv4.2 is highly expressed in the brain (PMID: 10551270). It is a major constituent of A-type potassium currents and a key regulator of neuronal membrane excitability (PMID: 22539834).

Protocols

Product Specific Protocols
WB protocol for Kv4.2 antibody 21298-1-APDownload protocol
IHC protocol for Kv4.2 antibody 21298-1-APDownload protocol
IF protocol for Kv4.2 antibody 21298-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Neurobiol Dis

MicroRNA inhibition upregulates hippocampal A-type potassium current and reduces seizure frequency in a mouse model of epilepsy.

Authors - Durgesh Tiwari
mouseWB

Sci Rep

Mir324 knockout regulates the structure of dendritic spines and impairs hippocampal long-term potentiation

Authors - Emma V Parkins
mouseWB

eNeuro

Estradiol- and Progesterone-Associated Changes in microRNA-Induced Silencing and Reduced Antiseizure Efficacy of an Antagomir in Female Mice

Authors - Durgesh Tiwari
ratWB,IHC,IF

JCI Insight

Indoxyl sulfate reduces Ito,f by activating ROS/MAPK and NF-κB signaling pathways.

Authors - Jing Yang
ratWB

Biochem Biophys Res Commun

Pinocembrin alleviates the susceptibility to atrial fibrillation in isoproterenol-induced rats

Authors - Zhangchi Liu
mouseWB

Res Sq

Age-dependent regulation of dendritic spine density and protein expression in Mir324 KO mice

Authors - Emma V Parkins