Kir7.1 Polyclonal antibody

Kir7.1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12657-1-AP

Synonyms

KCNJ13, KIR1.4, Kir7.1, SVD

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Validation Data Gallery

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  WB analysis of human brain using 12657-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12657-1-AP (Kir7.1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12657-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12657-1-AP (Kir7.1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 12657-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12657-1-AP (Kir7.1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 12657-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12657-1-AP (Kir7.1 antibody at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, human kidney tissue
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

12657-1-AP targets Kir7.1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Kir7.1 fusion protein Ag3327
• Full Name: potassium inwardly-rectifying channel, subfamily J, member 13
• Calculated Molecular Weight: 360 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC037290
• Gene Symbol: KCNJ13
• Gene ID (NCBI): 3769
• RRID: AB_2130488
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kir7.1, encoded by KCNJ13 gene, is a member of the inwardly rectifying potassium channel family of proteins. Kir7.1 is a low-conductance channel. At mRNA level, Kir7.1 is predominantly expressed in small intestine and also expressed in stomach, kidney, and brain. Mutations in KCNJ13 gene are associated with snowflake vitreoretinal degeneration.

Publications

Species	Application	Title
human	IHC	Acta Biochim Biophys Sin (Shanghai); Identification of lipid metabolism-related genes as prognostic indicators in papillary thyroid cancer.; Authors - Shishuai Wen