Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HuH-7 cells, Jurkat cells, K-562 cells, MCF-7 cells, U2OS cells |
| Positive IP detected in | HuH-7 cells |
| Positive IHC detected in | human breast cancer tissue, human intrahepatic cholangiocarcinoma tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27279-1-AP targets KCTD10 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25971 Product name: Recombinant human KCTD10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 253-313 aa of BC040062 Sequence: TLNILLYEAQDGRGPDNALLEATGGAAGRSHHLDEDEERERIERVRRIHIKRPDDRAHLHQ Predict reactive species |
| Full Name | potassium channel tetramerisation domain containing 10 |
| Calculated Molecular Weight | 313 aa, 35 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC040062 |
| Gene Symbol | KCTD10 |
| Gene ID (NCBI) | 83892 |
| RRID | AB_2880827 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H3F6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KCTD10 is a member of the polymerase delta-interacting protein 1 (PDIP1) gene family, which consists of 3 members, PDIP1, KCTD10 and TNFAIP1. KCTD10 is inducible by TNF-α, interacts with PCNA and the small subunit (p50) of DNA polymerase δ. Previous reports have shown that KCTD10 is essential for mammalian development. Further functional studies showed that KCTD10 interacts with proliferating cell nuclear antigen (PCNA) and plays an important role in DNA synthesis.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for KCTD10 antibody 27279-1-AP | Download protocol |
| WB protocol for KCTD10 antibody 27279-1-AP | Download protocol |
| IHC protocol for KCTD10 antibody 27279-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KCTD10 Polyclonal antibody (catalog number 27279-1-AP) has 9 total citations. These appear in journals including Nature Communications, Molecular Cell, International Journal of Biological Sciences, British Journal of Pharmacology, Frontiers in Immunology, Frontiers in Cell and Developmental Biology, Toxicology and Applied Pharmacology, and Genetics Research. The associated research fields span ubiquitination, innate immunity, cancer (lung, breast, hepatocellular), ferroptosis, angiogenesis, and diabetic retinopathy.
| Species | Application | Title |
|---|---|---|
Nat Commun RHOA regulates mitochondria-ER contact sites through modulation of the VAPB/PTPIP51 tether. | ||
Nat Commun Regulation of TRIF-mediated innate immune response by K27-linked polyubiquitination and deubiquitination.
| ||
Int J Biol Sci Aryl Hydrocarbon Receptor (AHR) Suppresses Arsenic (As3+)-Induced Malignant Transformation by Antagonizing TOX Expression
| ||
Br J Pharmacol KCTD10 weakens m6A modification of IL-11 in a ubiquitin-dependent manner to repress tumour immune escape in lung adenocarcinoma. | ||
Front Immunol KCTD10 inhibits lung cancer metastasis and angiogenesis via ubiquitin-mediated β-catenin degradation. |
Reviews
What customers say
Reviews for this antibody are mixed. One user reported it performs well for Western blot and immunohistochemistry at a 1:1000 dilution on prostate and breast cancer cells, giving it a top rating. Another user found it suitable for immunofluorescence and immunoblotting on breast and cervical cancer cells but noted it was ineffective for ChIP, flow cytometry, and other applications, resulting in a low rating. Overall, the antibody appears reliable for WB and IHC at the recommended dilution, but performance in other applications may be inconsistent.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Vasu (Verified Customer) (09-12-2025) | This antibody is suitable for few applications like immunofluroscence and Immunoblotting but not for other applications like Chip and flow cytometry and other applications
|
Vasudevarao (Verified Customer) (09-04-2025) | This antibody works very well for immunoblotting and IHC at the recommended dilutions
|
