Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20813-1-AP targets KDM1 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14778 Product name: Recombinant human KDM1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 524-876 aa of BC040194 Sequence: TQGKLEEKLQELEANPPSDVYLSSRDRQILDWHFANLEFANATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVALAEGLDIKLNTAVRQVRYTASGCEVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLNKVVLCFDRVFWDPSVNLFGHVGSTTASRGELFLFWNLYKAPILLALVAGEAAGIMENISDDVIVGRCLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSGNDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIADQFLGAMYTLPRQATPGVPAQQSPSM Predict reactive species |
| Full Name | lysine (K)-specific demethylase 1 |
| Calculated Molecular Weight | 876 aa, 95 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC040194 |
| Gene Symbol | KDM1 |
| Gene ID (NCBI) | 23028 |
| RRID | AB_10697809 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60341 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Lysine specific demethylase 1 (LSD1/BHC110/KIAA0601/p110b/AOF2/KDM1) is an amine oxidase that catalyzes histone demethylation via a flavin adenine dinucleotide (FAD)-dependent oxidative reaction(PMID:19703393). This protein belongs to the flavin monoamine oxidase family. Its structure and function is conserved from yeast to human and it is typically associated to CoREST, a corepressor protein, and histone deacetylases HDAC1 and HDAC2. Human LSD1 consists of 852 amino acids and comprises an N-terminal SWIRM domain, involved in protein interactions, and a C-terminal amine oxidase domain, which contains an insertion that forms the CoREST interacting site (the so-called tower domain )(PMID:20164337). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 93-95 kDa and apparent molecular mass of 110 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KDM1 antibody 20813-1-AP | Download protocol |
| IP protocol for KDM1 antibody 20813-1-AP | Download protocol |
| WB protocol for KDM1 antibody 20813-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-LSD1 antibody (Cat# 20813-1-AP) has 16 citations in journals including Cancer Research, Oncogene, EMBO Journal, Cell Reports, Advanced Science, Journal of Biological Chemistry, iScience, International Journal of Molecular Sciences, Cancer Biology & Medicine, Molecules, Environmental Toxicology, Immunology Research, Cancer Cell International, Advanced Biology, and International Journal of Legal Medicine. Research fields span epigenetics, chromatin remodeling, liver/lung/colon cancer, glioblastoma, neuroblastoma, osteoarthritis, rheumatoid arthritis, and adipose thermogenesis.
| Species | Application | Title |
|---|---|---|
Cancer Res HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc. | ||
Adv Sci (Weinh) PWWP2B Fine-Tunes Adipose Thermogenesis by Stabilizing HDACs in a NuRD Subcomplex. | ||
Cancer Biol Med HBx-mediated GPT2 suppression promotes liver cancer development by downregulating ADH1A. | ||
Oncogene Dual function of DOT1L suppresses tumor cell-intrinsic immunogenicity in hepatocellular carcinoma. | ||
Cell Rep E3 ligase Trim35 inhibits LSD1 demethylase activity through K63-linked ubiquitination and enhances anti-tumor immunity in NSCLC
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Reviews
What customers say
The single review for this product (used in Western Blot on HeLa cells at a 1:8000 dilution) rates it 2 out of 5 stars. The reviewer found the antibody acceptable for detecting total protein extracts but noted it performs poorly for assessing the purity of mitochondrial or nuclear isolations, recommending it only for general total-extract applications rather than fractionation quality control.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Veronica (Verified Customer) (06-03-2025) | Needs improvement. Can work for total extrct but I won' recommend it for testing mitochondria isolation purity or nuclei isolation.
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