KDM1 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 67037-1-Ig (same clone as 67037-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67037-1-Ig (KDM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

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  WB analysis using 67037-1-Ig (same clone as 67037-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67037-1-Ig (KDM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67037-1-Ig (same clone as 67037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67037-1-Ig (same clone as 67037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67037-1-Ig (same clone as 67037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67037-1-Ig (same clone as 67037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67037-1-Ig (same clone as 67037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67037-1-Ig (same clone as 67037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67037-1-Ig (same clone as 67037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51540-1

  Cytometric bead array sample test of MP51540-1, KDM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67037-1-PBS. Detection antibody: 67037-2-PBS.

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  Cytometric bead array standard curve of MP51540-1

  Cytometric bead array standard curve of MP51540-1, KDM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67037-1-PBS. Detection antibody: 67037-2-PBS. Standard:Ag14778. Range: 3.125-400 ng/mL.

KDM1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 67037-1-PBS

Clone No.2F10A12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

LSD1, [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A, 2F10A12, AOF 2, AOF2

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Product Information

67037-1-PBS targets KDM1 as part of a matched antibody pair:

MP51540-1: 67037-1-PBS capture and 67037-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14778

  Product name: Recombinant human KDM1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 524-876 aa of BC040194

  Sequence: TQGKLEEKLQELEANPPSDVYLSSRDRQILDWHFANLEFANATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVALAEGLDIKLNTAVRQVRYTASGCEVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLNKVVLCFDRVFWDPSVNLFGHVGSTTASRGELFLFWNLYKAPILLALVAGEAAGIMENISDDVIVGRCLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSGNDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIADQFLGAMYTLPRQATPGVPAQQSPSM

• Full Name: lysine (K)-specific demethylase 1
• Calculated Molecular Weight: 876 aa, 95 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC040194
• Gene Symbol: KDM1
• Gene ID (NCBI): 23028
• RRID: AB_2882351
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O60341
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Lysine specific demethylase 1 (LSD1/BHC110/KIAA0601/p110b/AOF2/KDM1) is an amine oxidase that catalyzes histone demethylation via a flavin adenine dinucleotide (FAD)-dependent oxidative reaction(PMID:19703393). This protein belongs to the flavin monoamine oxidase family. Its structure and function is conserved from yeast to human and it is typically associated to CoREST, a corepressor protein, and histone deacetylases HDAC1 and HDAC2. Human LSD1 consists of 852 amino acids and comprises an N-terminal SWIRM domain, involved in protein interactions, and a C-terminal amine oxidase domain, which contains an insertion that forms the CoREST interacting site (the so-called tower domain )(PMID:20164337). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 93-95 kDa and apparent molecular mass of 110 kDa.