KDM2A Polyclonal antibody

KDM2A Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 24311-1-AP

Synonyms

CXXC8, F box protein FBL7, F box protein Lilina, F box/LRR repeat protein 11, FBL11, FBL7, FBXL11, JHDM1A, KDM2A, KIAA1004, LILINA, lysine (K) demethylase 2A, Lysine specific demethylase 2A



Tested Applications

Positive WB detected inJurkat cells, NIH/3T3 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24311-1-AP is KDM2A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KDM2A fusion protein Ag19448
Full Name lysine (K)-specific demethylase 2A
Calculated Molecular Weight 1162 aa, 133 kDa
Observed Molecular Weight 126 kDa
GenBank Accession NumberBC064360
Gene Symbol KDM2A
Gene ID (NCBI) 22992
RRIDAB_2879488
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KDM2A(Lysine-specific demethylase 2A) is also named as CXXC8, FBL7, FBXL11, JHDM1A, KIAA1004 and belongs to the JHDM1 histone demethylase family. It is required to maintain the heterochromatic state, and playing a role in epigenetic silencing. KDM2A is one of the four subunits of the E3 ubiquitin protein ligase complex (SCF) with SKP1, CUL1, RBX1, Fbox protein bringing ubiquitin conjugating enzymes to substrates recruited by F-box.

Protocols

Product Specific Protocols
WB protocol for KDM2A antibody 24311-1-APDownload protocol
IP protocol for KDM2A antibody 24311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,CoIP

Cell Oncol (Dordr)

Hepatitis B virus core protein stabilizes RANGAP1 to upregulate KDM2A and facilitate hepatocarcinogenesis

Authors - Hong-Juan You
humanWB

J Cell Mol Med

Circular RNA circFOXO3 regulates KDM2A by targeting miR-214 to promote tumor growth and metastasis in oral squamous cell carcinoma.

Authors - Yilong Ai
humanWB

J Cell Physiol

The Ubiquitin Ligase SCF(FBXW7α) Promotes ATA3 Degradation.

Authors - Nan Song
humanIP, WB

Cell Rep

Enhancer RNAs Mediate Estrogen-Induced Decommissioning of Selective Enhancers by Recruiting ERα and Its Cofactor.

Authors - Mei Yang
humanWB,IHC

Cell Death Dis

CircRNF144B/miR-342-3p/FBXL11 axis reduced autophagy and promoted the progression of ovarian cancer by increasing the ubiquitination of Beclin-1

Authors - Wenting Song