KDM5C Recombinant antibody, PBS Only

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  WB analysis using 86017-1-RR (same clone as 86017-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86017-1-RR (KDM5C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86017-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86017-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86017-1-RR against Human KDM5C were performed. The affinity constant is 0.148 nM.

KDM5C Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 86017-1-PBS

Clone No.250484C10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

Synonyms

[histone H3]-trimethyl-L-lysine(4) demethylase 5C, DXS1272E, EC:1.14.11.67, JARID1C, Jumonji/ARID domain-containing protein 1C

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Product Information

86017-1-PBS targets KDM5C in applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KDM5C fusion protein Ag5791
• Full Name: lysine (K)-specific demethylase 5C
• Calculated Molecular Weight: 176 kDa
• Observed Molecular Weight: 171-180 kDa
• GenBank Accession Number: BC054499
• Gene Symbol: KDM5C
• Gene ID (NCBI): 8242
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P41229
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

KDM5C belongs to the ARID protein family that harbors conserved DNA-binding motifs and acts as a histone H3 lysine 4 demethylase, suggesting a potential role in epigenetic regulation during development, cell differentiation (PMID:21575681). KDM5C protein is homologous to three other proteins of the human JARID1 family (JARID1A, JARID1B and JARID1C). This family shares several evolutionarily conserved domains, including the catalytic JmjC domain, JmjN domain, Arid/Bright domain, C5HC2 zinc-finger domain and two plant homeodomains (PHD), a motif that binds methyl-lysine residues (PMID:17320160,17320161).