KDM5C Recombinant antibody

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  WB analysis using 86017-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86017-1-RR (KDM5C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86017-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86017-1-RR against Human KDM5C were performed. The affinity constant is 0.148 nM.

KDM5C Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86017-1-RR

Clone No.250484C10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

Synonyms

[histone H3]-trimethyl-L-lysine(4) demethylase 5C, DXS1272E, EC:1.14.11.67, JARID1C, Jumonji/ARID domain-containing protein 1C

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, MCF-7 cells, HEK-293 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86017-1-RR targets KDM5C in applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KDM5C fusion protein Ag5791
• Full Name: lysine (K)-specific demethylase 5C
• Calculated Molecular Weight: 176 kDa
• Observed Molecular Weight: 171-180 kDa
• GenBank Accession Number: BC054499
• Gene Symbol: KDM5C
• Gene ID (NCBI): 8242
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P41229
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KDM5C belongs to the ARID protein family that harbors conserved DNA-binding motifs and acts as a histone H3 lysine 4 demethylase, suggesting a potential role in epigenetic regulation during development, cell differentiation (PMID:21575681). KDM5C protein is homologous to three other proteins of the human JARID1 family (JARID1A, JARID1B and JARID1C). This family shares several evolutionarily conserved domains, including the catalytic JmjC domain, JmjN domain, Arid/Bright domain, C5HC2 zinc-finger domain and two plant homeodomains (PHD), a motif that binds methyl-lysine residues (PMID:17320160,17320161).