KHDC1 Polyclonal antibody

KHDC1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23443-1-AP

Synonyms

C6orf148, KHDC1, NDG1

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Validation Data Gallery

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  WB analysis of L02 using 23443-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 23443-1-AP (KHDC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 23443-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 23443-1-AP (KHDC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 23443-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 23443-1-AP (KHDC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach using 23443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 23443-1-AP (KHDC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 23443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 23443-1-AP (KHDC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of L02 using 23443-1-AP

  Immunofluorescent analysis of L02 cells using 23443-1-AP (KHDC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: L02 cells
• Positive IHC detected in: human liver tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23443-1-AP is KHDC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KHDC1 fusion protein Ag20115
• Full Name: KH homology domain containing 1
• Calculated Molecular Weight: 237 aa, 27 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC022080
• Gene Symbol: KHDC1
• Gene ID (NCBI): 80759
• RRID: AB_2879280
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KHDC1, also named as C6orf148, belongs to the KHDC1 family. KHDC1 has two isoforms with calculated MW 27 kDa and 18 kDa. It's a membrane protein. We got 35-40 kDa in our WB detection.