KHK Polyclonal antibody

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KHK Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15681-1-AP Copied!

Publications (15)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:2.7.1.3, Ketohexokinase

• 

  WB analysis using 15681-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 15681-1-AP

  WB result of KHK antibody (15681-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-KHK transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 15681-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 15681-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 15681-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse liver using 15681-1-AP

  IP result of anti-KHK (IP:15681-1-AP, 4ug; Detection:15681-1-AP 1:500) with mouse liver tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 15681-1-AP

  IP result of anti-KHK (IP:15681-1-AP, 4ug; Detection:15681-1-AP 1:1000) with HepG2 cells lysate 960 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 15681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15681-1-AP (KHK Antibody) at dilution of 1:400 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 15681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15681-1-AP (KHK Antibody) at dilution of 1:400 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 15681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15681-1-AP (KHK Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 15681-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15681-1-AP (KHK Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 15681-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 15681-1-AP (KHK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 15681-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using KHK antibody (15681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells, human liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue, HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15681-1-AP targets KHK in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8182

  Product name: Recombinant human KHK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-298 aa of BC006233

  Sequence: MEEKQILCVGLVVLDVISLVDKYPKEDSEIRCLSQRWQRGGNASNSCTILSLLGAPCAFMGSMAPGHVADFVLDDLRRYSVDLRYTVFQTTGSVPIATVIINEASGSRTILYYDRSLPDVSATDFEKVDLTQFKWIHIEGRNASEQVKMLQRIDAHNTRQPPEQKIRVSVEVEKPREELFQLFGYGDVVFVSKDVAKHLGFQSAEEALRGLYGRVRKGAVLVCAWAEEGADALGPDGKLLHSDAFPPPRVVDTLGAGDTFNASVIFSLSQGRSVQEALRFGCQVAGKKCGLQGFDGIV

• Full Name: ketohexokinase (fructokinase)
• Calculated Molecular Weight: 298 aa, 33 kDa
• Observed Molecular Weight: 33-35 kDa
• GenBank Accession Number: BC006233
• Gene Symbol: KHK
• Gene ID (NCBI): 3795
• RRID: AB_2130496
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P50053
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ketohexokinase catalyzes conversion of fructose to fructose-1-phosphate, it is the first enzyme with a specialized pathway that catabolizes dietary fructose. Fructose metabolism seems to provide cancer cells with the supplementary fuel required for proliferation and metastasis.

Publications

What published studies show

The KHK (Ketohexokinase) polyclonal antibody (Cat# 15681-1-AP) has 15 citations published in journals including Signal Transduction and Targeted Therapy, Cell Metabolism, Nature Metabolism, Advanced Science, Brain, European Journal of Pharmacology, Chemical Biology & Interactions, Cancer Science, Molecular Nutrition & Food Research, FASEB Journal, Endocrinology, PeerJ, Biochemical & Biophysical Research Communications, and Biochemical Pharmacology. Research fields span fructose metabolism, hepatocellular carcinoma, cervical cancer, pancreatic cancer, breast cancer, glioma, ALS, hyperuricemia, glucose homeostasis, and cardiac hypertrophy.

Automatically generated from the 15 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Signal Transduct Target Ther; Fructose 1-phosphate inhibits mannose phosphate isomerase to suppress hepatocellular carcinogenesis; Authors - Yongqiang Wang
mouse	WB,IHC	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
mouse	WB	Nat Metab; The small intestine shields the liver from fructose-induced steatosis.; Authors - Cholsoon Jang
human	WB	Adv Sci (Weinh); NAT10/ac4C/FOXP1 Promotes Malignant Progression and Facilitates Immunosuppression by Reprogramming Glycolytic Metabolism in Cervical Cancer; Authors - Xiaona Chen
human	WB	Brain; C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.; Authors - Scott P Allen
	WB	Eur J Pharmacol; Oxyberberine alleviates fructose-induced hyperuricemia by modulating purine metabolism and gut microbiota; Authors - Wenwen Tan