KIAA0528 Polyclonal antibody, PBS Only

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• KIAA0528 Polyclonal antibody, PBS Only (21138-1-PBS)

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  WB analysis of HeLa using 21138-1-AP (same clone as 21138-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

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  WB analysis using 21138-1-AP (same clone as 21138-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 21138-1-AP (same clone as 21138-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 21138-1-AP (same clone as 21138-1-PBS)

  IP result of anti-KIAA0528 (IP:21138-1-AP, 4ug; Detection:21138-1-AP 1:700) with HeLa cells lysate 520ug. This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 21138-1-AP (same clone as 21138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 21138-1-AP (same clone as 21138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 21138-1-AP (same clone as 21138-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using KIAA0528 antibody 21138-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21138-1-PBS in a different storage buffer formulation.

KIAA0528 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 21138-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CDP138, C2CD5, C2 domain-containing protein 5, C2 domain-containing phosphoprotein of 138 kDa

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Product Information

21138-1-PBS targets KIAA0528 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15590

  Product name: Recombinant human KIAA0528 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 651-1000 aa of BC042498

  Sequence: RSRLLRSQSESSDEVTELDLSHGKKDAFVLEIDDTDAMEDVHSLLTDVPPPSGFYSCNTEIMPGINNWTSEIQMFTSVRVIRLSSLNLTNQALNKNFNDLCENLLKSLYFKLRSMIPCCLCHVNFTVSLPEDELIQVTVTAVAITFDKNQALQTTKTPVEKSLQRASTDNEELLQFPLELCSDSLPSHPFPPAKAMTVEKASPVGDGNFRNRSAPPCANSTVGVVKMTPLSFIPGAKITKYLGIINMFFIRETTSLREEGGVSGFLHAFIAEVFAMVRAHVAALGGNAVVSYIMKQCVFMENPNKNQAQCLINVSGDAVVFVRESDLEVVSSQQPTTNCQSSCTEGEVTT

• Full Name: KIAA0528
• Calculated Molecular Weight: 1000 aa, 110 kDa
• Observed Molecular Weight: 110-125 kDa
• GenBank Accession Number: BC042498
• Gene Symbol: KIAA0528
• Gene ID (NCBI): 9847
• RRID: AB_10858480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86YS7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.