Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KIAA0776 antibody (83047-5-RR, Clone: 230363G7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KIAA0776 antibody (83047-5-RR, Clone: 230363G7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).
FC experiment of U2OS using 83047-5-RR
1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human KIAA0776 (83047-5-RR, Clone:230363G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human KIAA0776 (83047-5-RR, Clone:230363G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 83047-5-RR
Biolayer interferometry (BLl) kinetic assays of 83047-5-RR
against Human KIAA0776 were performed. The affinity constant is below 1 pM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KIAA0776 antibody (83047-5-RR, Clone: 230363G7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).
FC (INTRA) Figures
FC experiment of U2OS using 83047-5-RR
1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human KIAA0776 (83047-5-RR, Clone:230363G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
AFFINITY Figures
Affinity and Kinetic Characterization of 83047-5-RR
Biolayer interferometry (BLl) kinetic assays of 83047-5-RR
against Human KIAA0776 were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KIAA0776 Recombinant antibody
Catalog Number
83047-5-RR
Citations
-
Dilutions
IF/ICC : 1:125-1:500 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
IF/ICC, FC (Intra), ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
