KIAA1199 / CEMIP Polyclonal antibody

• Featured Product
• KD/KO Validated

KIAA1199 / CEMIP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

47

Reviews (1)

Cat no : 21129-1-AP

Synonyms

KIAA1199, CEMIP, Protein KIAA1199, HYBID, Hyaluronan binding protein involved in hyaluronan depolymerization

---

Validation Data Gallery

• 

  WB analysis using 21129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21129-1-AP (KIAA1199 / CEMIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21129-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21129-1-AP (KIAA1199 / CEMIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate hyperplasia using 21129-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 21129-1-AP (KIAA1199 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 21129-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 21129-1-AP (KIAA1199 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 21129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21129-1-AP (KIAA1199 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse testis using 21129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21129-1-AP (KIAA1199 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 21129-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 21129-1-AP (KIAA1199 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 21129-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 21129-1-AP (KIAA1199 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach cancer using 21129-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 21129-1-AP (CEMIP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 21129-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 21129-1-AP (CEMIP antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 21129-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KIAA1199 / CEMIP antibody (21129-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 21129-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug KIAA1199 / CEMIP Polyclonal antibody (21129-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: U-87 MG cells, U2OS cells, COLO 320 cells, SGC-7901 cells
• Positive IHC detected in: human prostate hyperplasia tissue, mouse testis tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 18 publications below
• WB: See 32 publications below
• IHC: See 28 publications below
• IF: See 9 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Product Information

21129-1-AP targets KIAA1199 / CEMIP in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, marmoset
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIAA1199 / CEMIP fusion protein Ag15527
• Full Name: KIAA1199 / CEMIP
• Calculated Molecular Weight: 1361 aa, 153 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC020256
• Gene Symbol: KIAA1199
• Gene ID (NCBI): 57214
• RRID: AB_10695771
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA1199, also known as CEMIP, was first discovered as an inner-ear protein whose genetic mutations were linked to nonsyndromic hearing loss. KIAA1199 is involved in hyaluronan degradation. Studies have shown that KIAA1199 is overexpressed in many types of cancers, suggesting a critical role of KIAA1199 in cancer progression.

Publications

Species	Application	Title
human	WB	Nat Commun; TREX tetramer disruption alters RNA processing necessary for corticogenesis in THOC6 Intellectual Disability Syndrome; Authors - Elizabeth A Werren
human	IHC	Sci Adv; Single-cell analysis of human basal cell carcinoma reveals novel regulators of tumor growth and the tumor microenvironment.; Authors - Christian F Guerrero-Juarez
human	WB	Am J Hum Genet; Altered regulation of DPF3, a member of the SWI/SNF complexes, underlies the 14q24 renal cancer susceptibility locus.; Authors - Leandro M Colli ; KD Validated
human	IHC	Theranostics; Functional Genomic mRNA Profiling of Colorectal Adenomas: Identification and in vivo Validation of CD44 and Splice Variant CD44v6 as Molecular Imaging Targets.; Authors - Elmire Hartmans
human	IF	Cell Rep; Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss.; Authors - Makoto Hosoya
human	WB,IHC,IF,IP	Oncogene; KIAA1199 promotes metastasis of colorectal cancer cells via microtubule destabilization regulated by a PP2A/stathmin pathway.; Authors - Lei Zhao ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

DAN (Verified Customer) (06-10-2022)

• Applications: Western Blot

Related Products

FAM3C Polyclonal antibody

14247-1-AP

Mu Crystallin Polyclonal antibody

12495-1-AP

MESDC2 Polyclonal antibody

10958-1-AP

NF-κB p65 Polyclonal antibody

10745-1-AP

N-cadherin Monoclonal antibody

66219-1-Ig