Various lysates were subjected to SDS PAGE followed by western blot with 83900-7-RR (KIAA1586 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 83900-7-RR (KIAA1586 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Affinity and Kinetic Characterization of 83900-7-RR
Biolayer interferometry (BLl) kinetic assays of 83900-7-RR against Human KIAA1586 were performed. The affinity constant is below 1 pM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
KIAA1586, close to ZNF451, encodes a protein with similar N-terminal tandem SIMs, which has also shown elongase activity. ZNF451 is an E3 SUMO-protein ligase that facilitates UBE2I/UBC9-mediated SUMO2 modification of target proteins (PMID: 26524493, PMID: 35887358).
Various lysates were subjected to SDS PAGE followed by western blot with 83900-7-RR (KIAA1586 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
AFFINITY Figures
Affinity and Kinetic Characterization of 83900-7-RR
Biolayer interferometry (BLl) kinetic assays of 83900-7-RR against Human KIAA1586 were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KIAA1586 Recombinant antibody
Catalog Number
83900-7-RR
Citations
-
Dilutions
WB : 1:500-1:2000
Applications
WB, ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
