KIAA1586 Recombinant monoclonal antibody

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  WB analysis using 83900-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83900-7-RR (KIAA1586 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83900-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83900-7-RR against Human KIAA1586 were performed. The affinity constant is below 1 pM.

KIAA1586 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 83900-7-RR

Clone No.240972F10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

240972F10

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83900-7-RR targets KIAA1586 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag36038

  Product name: Recombinant human KIAA1586 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 426-749 aa of BC041125

  Sequence: SLDDSISEIKQINHLKIFIDKIYSIYHQPNKNQTKLLGTVAKELETEIIKIGRVMGPRWAACSLQAATAVWHAYPILYMHFSHSYSGLAKRLANINFLQDLALMIDILEEFSVLSTALQSRSTNIKKAQKLIKRTIRALENLKIGTGKYESQIEDLIKSDKFKDIPFNKNNKFNALPRSILLDNIIQHMNLRLLSDRNHEDIFNYFDLLEPSTWPYEEITSPWIAGEKTLFHLCKILKYEVDLNDFREFVNNNIKSNNVSIPTTIYKAKKIVSTIAINSAEAERGFNLMNIICTRVRNSLTIDHVSDLMTINLLGKELADWDAT

• Full Name: KIAA1586
• Calculated Molecular Weight: 787 aa, 90 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC041125
• Gene Symbol: KIAA1586
• Gene ID (NCBI): 57691
• RRID: AB_3671481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9HCI6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA1586, close to ZNF451, encodes a protein with similar N-terminal tandem SIMs, which has also shown elongase activity. ZNF451 is an E3 SUMO-protein ligase that facilitates UBE2I/UBC9-mediated SUMO2 modification of target proteins (PMID: 26524493, PMID: 35887358).