KIAA1715 Recombinant monoclonal antibody

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  WB analysis using 83418-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83418-4-RR (KIAA1715 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 83418-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83418-4-RR (KIAA1715 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of U-251 using 83418-4-RR

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug KIAA1715 Recombinant antibody (83418-4-RR, Clone:240211C7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

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  Affinity and Kinetic Characterization of 83418-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83418-4-RR against Human KIAA1715 were performed. The affinity constant is 13.1 nM.

KIAA1715 Uni-rAb^® Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 83418-4-RR

Clone No.240211C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

LNP, 240211C7, ER junction formation factor lunapark, LNPK, Protein lunapark

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse kidney tissue
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83418-4-RR targets KIAA1715 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag34669

  Product name: Recombinant human KIAA1715 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 236-428 aa of BC105132

  Sequence: GLHPPGPPLARPILPRERGALDRIVEYLVGDGPQNRYALICQQCFSHNGMALKEEFEYIAFRCAYCFFLNPARKTRPQAPRLPEFSFEKRQVVEGSSSVGPLPSGSVLSSDNQFNEESLEHDVLDDNTEQTDDKIPATEQTNQVIEKASDSEEPEEKQETENEEASVIETNSTVPGADSIPDPELSGESLTAE

• Full Name: KIAA1715
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC105132
• Gene Symbol: KIAA1715
• Gene ID (NCBI): 80856
• RRID: AB_3671066
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9C0E8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA1715, also known as LNPK, is a member of the conserved Lunapark protein family. KIAA1715 is an endoplasmic reticulum (ER) membrane protein consisting of an N-terminal domain important for N-myristoylation and ER targeting, two transmembrane domains and a unique zinc finger domain. It has 4 isoforms produced by alternative splicing and acts mainly as a stabilizer of negative membrane curvature (PMID: 27619977).