Eg5 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 84966-2-RR (same clone as 84966-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84966-2-RR (Eg5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84966-2-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 84966-2-RR (same clone as 84966-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84966-2-RR (Eg5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84966-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84966-2-RR (same clone as 84966-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Eg5 antibody (84966-2-RR, Clone: 242611D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84966-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84966-2-RR (same clone as 84966-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Eg5 Recombinant antibody (84966-2-RR, Clone:242611D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84966-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01688-2

  Cytometric bead array standard curve of MP01688-2, Eg5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84966-2-PBS. Detection antibody: 84966-1-PBS. Standard: Ag20001. Range: 3.125-100 ng/mL.

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  Affinity and Kinetic Characterization of 84966-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84966-2-RR against Human Eg5 were performed. The affinity constant is 18.3 nM.

Eg5 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 84966-2-PBS

Clone No.242611D10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

KIF11, 242611D10, Kinesin-related motor protein Eg5, Kinesin-like spindle protein HKSP, Kinesin-like protein KIF11

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Product Information

84966-2-PBS targets Eg5 as part of a matched antibody pair:

MP01688-2: 84966-2-PBS capture and 84966-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Eg5 fusion protein Ag20001
• Full Name: kinesin family member 11
• Calculated Molecular Weight: 1056 aa, 119 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC136474
• Gene Symbol: Eg5
• Gene ID (NCBI): 3832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P52732
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Eg5 is a plus-end-directed microtubule motor encoded by KIF11 gene. It is an essential mitotic kinesin that plays a pivotal role in the formation and maintenance of bipolar spindle during early prometaphase. Eg5 has been implicated in tumourigenesis, and its overexpression has been found in various cancer tissues.