KIF13B Polyclonal antibody

KIF13B Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 30141-1-AP

Synonyms

GAKIN, KIAA0639, KIF13B, kinesin family member 13B, Kinesin like protein GAKIN, Kinesin like protein KIF13B

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Validation Data Gallery

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  WB analysis using 30141-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30141-1-AP (KIF13B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 30141-1-AP

  IP result of anti-KIF13B (IP:30141-1-AP, 4ug; Detection:30141-1-AP 1:800) with HEK-293 cells lysate 1400 ug.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30141-1-AP targets KIF13B in WB, IP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF13B fusion protein Ag30876
• Full Name: kinesin family member 13B
• Calculated Molecular Weight: 203 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: NM_015254
• Gene Symbol: KIF13B
• Gene ID (NCBI): 23303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF13B, Also known as GAKIN, is a 250-kD phosphoprotein. KIF13B contains a consensus ATP/GTP-binding motif within its N-terminal motor domain; a stalk domain containing several short alpha-helical segments; and a CAP-gly motif in its C terminus characteristic of microtubule-based cytoskeleton components(PMID: 10859302).