KIF17 Polyclonal antibody

KIF17 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14615-1-AP

Synonyms

KIAA1405, KIF17, KIF17B, KIF3 related motor protein, KIF3X, kinesin family member 17, Kinesin like protein KIF17

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Validation Data Gallery

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  WB analysis using 14615-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14615-1-AP (KIF17 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14615-1-AP

  IP result of anti-KIF17 (IP:14615-1-AP, 4ug; Detection:14615-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14615-1-AP (KIF17 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14615-1-AP (KIF17 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14615-1-AP (KIF17 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14615-1-AP (KIF17 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14615-1-AP (KIF17 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 14615-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14615-1-AP (KIF17 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of COS-7 using 14615-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed COS-7 cells using 14615-1-AP (KIF17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, human brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: COS-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

14615-1-AP targets KIF17 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF17 fusion protein Ag6217
• Full Name: kinesin family member 17
• Calculated Molecular Weight: 115 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC065927
• Gene Symbol: KIF17
• Gene ID (NCBI): 57576
• RRID: AB_2131278
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Dev Cell; ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.; Authors - Jie Ran
human	WB,IF,IP	Neurosci Bull; KIF17 Modulates Epileptic Seizures and Membrane Expression of the NMDA Receptor Subunit NR2B.; Authors - Yan Liu
human	WB,IHC,IF,CoIP	Cancer Lett; KIF17 maintains the epithelial phenotype of breast cancer cells and curbs tumour metastasis; Authors - Tianfu Li ; KD Validated