KIF1A Polyclonal antibody

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• KIF1A Polyclonal antibody (24841-1-AP)

• 

  WB analysis using 24841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24841-1-AP (KIF1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 24841-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using KIF1A antibody (24841-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

KIF1A Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 24841-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

ATSV, C2orf20, HUNC 104, HUNC104, hUnc-104

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Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

24841-1-AP targets KIF1A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18817

  Product name: Recombinant human KIF1A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1508-1566 aa of BC111799

  Sequence: CPSLVEGRYGATDLRTPQPCSRPASPEPELLPEADSKKLPSPARATETDKEPQRLLVPD

• Full Name: kinesin family member 1A
• Calculated Molecular Weight: 1690 aa, 191 kDa
• Observed Molecular Weight: 191-203 kDa
• GenBank Accession Number: BC111799
• Gene Symbol: KIF1A
• Gene ID (NCBI): 547
• RRID: AB_3669455
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12756
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Autophagy; Feedback loops between DNMT1 and autophagy as well as senescence promotes organ aging and canities.; Authors - Lu Li
mouse	WB,IF	J Headache Pain; CREB-KIF1A-CGRP-positive feedback loop drives central sensitization in chronic migraine.; Authors - Wei Jiang ; KD Validated