Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human skeletal muscle tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15263-1-AP targets KIF1B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7263 Product name: Recombinant human KIF1B protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 230-579 aa of BC001415 Sequence: TSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRERLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSGDIESLVDREKELATKCLQLLTHTFNREFSQVHGSVSDCKLSDISPIGRDPSESSFSSATLTPSSTCPSLVDSRSNSLDQKTPEANSRASSPCPEFEQFQIVPAVETPYLARAGKNEFLNLVPDIEEIRPSSVVSKKGYLHFKEPLYSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDMNDWLYAFNPLLAGTIRSKLSRRCPSQSKY Predict reactive species |
| Full Name | kinesin family member 1B |
| Calculated Molecular Weight | 204 kDa |
| Observed Molecular Weight | 199 kDa, 204 kDa |
| GenBank Accession Number | BC001415 |
| Gene Symbol | KIF1B |
| Gene ID (NCBI) | 23095 |
| RRID | AB_2131412 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60333 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KIF1B antibody 15263-1-AP | Download protocol |
| IP protocol for KIF1B antibody 15263-1-AP | Download protocol |
| WB protocol for KIF1B antibody 15263-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KIF1B Polyclonal antibody (Cat# 15263-1-AP) has 9 citations. They appear in journals including Cell Reports, Cell Death & Disease, Journal of Hazardous Materials, Journal of Cell Biology, Experimental Neurology, Diabetes Metab Syndr Obes, Immunology Research, and eLife. Research fields span autophagy, axonal regeneration, mitochondrial transport, neuroinflammation, immune checkpoint regulation, and renal disease (diabetic nephropathy, IgA nephropathy).
| Species | Application | Title |
|---|---|---|
Cell Death Dis AAV-mediated inhibition of ULK1 promotes axonal regeneration in the central nervous system in vitro and in vivo. | ||
J Hazard Mater Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria. | ||
Cell Rep Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells.
| ||
Exp Neurol Axonal injury mediated by neuronal p75NTR/TRAF6/JNK pathway contributes to cognitive impairment after repetitive mTBI |
Reviews
What customers say
The single review for 15263-1-AP (KIF1B) reports a Western Blot on human primary fibroblasts using a 1:1000 dilution under reducing/denaturing conditions. The user loaded 15 µg of total protein on a 4–15% TGX gel, blocked with 3% BSA, incubated the primary antibody overnight at 4°C, and used a 1:5000 HRP secondary for 2 hours at room temperature. The reviewer rated the product 3 out of 5, noting that the blot showed many bands rather than a clean, specific signal. Overall, the feedback suggests the antibody works but may produce non-specific binding in this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Maria (Verified Customer) (07-31-2021) | KIF1B in human primary fibroblasts in culture. Reducing and denaturing conditions. 15 ug of total protein. TGX gel 4-15%. Blocking BSA 3%. 1:1000 primary ab O/N 4ºC. 1:5000 secondary HRP antibody 2h RT. Many bands in the blot.
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