KIF1C Polyclonal antibody

• Featured Product
• KD/KO Validated

KIF1C Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12760-1-AP

Synonyms

KIAA0706, KIF1C, kinesin family member 1C, Kinesin like protein KIF1C, LTXS1

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Validation Data Gallery

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  WB analysis of mouse heart using 12760-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12760-1-AP (KIF1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12760-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12760-1-AP (KIF1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 12760-1-AP

  IP result of anti-KIF1C (IP:12760-1-AP, 5ug; Detection:12760-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IHC staining of human gliomas using 12760-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12760-1-AP (KIF1C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12760-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12760-1-AP (KIF1C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, A549 cells, HeLa cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

12760-1-AP targets KIF1C in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF1C fusion protein Ag3500
• Full Name: kinesin family member 1C
• Calculated Molecular Weight: 1103 aa, 123 kDa
• Observed Molecular Weight: 135-140 kDa
• GenBank Accession Number: BC034993
• Gene Symbol: KIF1C
• Gene ID (NCBI): 10749
• RRID: AB_2131422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	RNA; The kinesin KIF1C transports APC-dependent mRNAs to cell protrusions.; Authors - Xavier Pichon ; KD Validated
mouse	WB,IF,IP	Nat Commun; Muscleblind-like proteins use modular domains to localize RNAs by riding kinesins and docking to membranes; Authors - Ryan P Hildebrandt
human		bioRxiv; A KIF1C-CNBP motor-adaptor complex for trafficking mRNAs to cell protrusions; Authors - Konstadinos Moissoglu