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KIF20A Monoclonal antibody

KIF20A Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A8E3

Cat no : 67190-1-Ig

Synonyms

FLJ21151, GG10_2, KIF20A, kinesin family member 20A, Kinesin like protein KIF20A, MKLP2, RAB6KIFL, Rabkinesin 6



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, HL-60 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67190-1-Ig targets KIF20A in WB, IHC, IF, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KIF20A fusion protein Ag8916
Full Name kinesin family member 20A
Calculated Molecular Weight 890 aa, 100 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC012999
Gene Symbol KIF20A
Gene ID (NCBI) 10112
RRIDAB_2882485
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF20A, also known as RAB6KIFL or MKlp2, is a kinesin originally identified as a binding partner for the rab6 GTPase involved in protein transport at the Golgi apparatus. KIF20A belongs to a large family of motor proteins that accumulate in mitotic cells and is required for normal cell division. KIF20A is thought to be involved in carcinogenesis. In normal tissues KIF20A is almost undetectable while it is overexpressed in various cancers. KIF20A has been identified as a novel promising candidate for anticancer immunotherapy.

Protocols

Product Specific Protocols
WB protocol for KIF20A antibody 67190-1-IgDownload protocol
IHC protocol for KIF20A antibody 67190-1-IgDownload protocol
IF protocol for KIF20A antibody 67190-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cancer Res

Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.

Authors - Bingteng Xie
mouse,humanWB,IF

Genomics

Upregulation of the key biomarker kinesin family member 20A (KIF20A) is associated with the pulmonary artery hypertension

Authors - Linqing Li
mouseIF

Res Sq

An oocyte meiotic midbody cap is required for developmental competence in mice

Authors - Karen Schindler
mouseIF

Nat Commun

An oocyte meiotic midbody cap is required for developmental competence in mice

Authors - Gyu Ik Jung
humanWB

Mol Cancer Res

Overlaid transcriptome and proteome analyses identify mitotic kinesins as important targets of arylsulfonamide-mediated RBM39 degradation

Authors - Seemon Coomar
humanWB

Mol Cancer Res

Overlaid Transcriptional and Proteome Analyses Identify Mitotic Kinesins as Important Targets of Arylsulfonamide-Mediated RBM39 Degradation

Authors - Seemon Coomar