KIF23 Polyclonal antibody

• Featured Product
• KD/KO Validated

KIF23 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 28587-1-AP

Synonyms

CHO1, KIF23, kinesin family member 23, Kinesin like protein 5, Kinesin like protein KIF23, KNSL5, Mitotic kinesin like protein 1, MKLP 1, MKLP1

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Validation Data Gallery

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  WB analysis using 28587-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28587-1-AP (KIF23 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 28587-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28587-1-AP (KIF23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 28587-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28587-1-AP (KIF23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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Tested Applications

• Positive WB detected in: HeLa cells, U-87 MG cells
• Positive IHC detected in: human stomach cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

28587-1-AP targets KIF23 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF23 fusion protein Ag29346
• Full Name: kinesin family member 23
• Calculated Molecular Weight: 110 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: NM_138555
• Gene Symbol: KIF23
• Gene ID (NCBI): 9493
• RRID: AB_2881176
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	PLoS Biol; The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.; Authors - Umut Batman
human	WB,IHC	Front Cell Dev Biol; Identification of KIF23 as a Prognostic Biomarker Associated With Progression of Clear Cell Renal Cell Carcinoma.; Authors - Zonglong Wu ; KD Validated
human	WB	Front Immunol; HBx Mediated Increase of DDX17 Contributes to HBV-Related Hepatocellular Carcinoma Tumorigenesis.; Authors - Mei-Ling Dong
human	WB	Toxicol Appl Pharmacol; Kinesin family member 23 exerts a protumor function in breast cancer via stimulation of the Wnt/β-catenin pathway.; Authors - Xin He ; KD Validated
	WB	Cell Rep; Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.; Authors - Ana C Almeida ; KD Validated