Various lysates were subjected to SDS PAGE followed by western blot with RMX00017 (KIF5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with RMX00017 (KIF5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IP experiment of U-87 MG using RMX00017
IP result of anti-KIF5A (IP:RMX00017, 4ug; Detection:RMX00017 1:4000) with U-87 MG cells lysate 1520 ug.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00017 (KIF5A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of PC-12 using RMX00017
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using KIF5A antibody (RMX00017) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using KIF5A antibody (RMX00017) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
SH-SY5Y cells, 87-MG cells, mouse brain tissue, rat brain tissue
Positive IP detected in
U-87 MG cells
Positive IHC detected in
mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
PC-12 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:10000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
RMX00017 targets KIF5A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.
Various lysates were subjected to SDS PAGE followed by western blot with RMX00017 (KIF5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using RMX00017
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00017 (KIF5A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using RMX00017
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00017 (KIF5A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of U-87 MG using RMX00017
IP result of anti-KIF5A (IP:RMX00017, 4ug; Detection:RMX00017 1:4000) with U-87 MG cells lysate 1520 ug.
IF/ICC Figures
IF Staining of PC-12 using RMX00017
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using KIF5A antibody (RMX00017) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KIF5A Multi-Recombinant antibody
Catalog Number
RMX00017
Citations
-
Dilutions
WB : 1:2000-1:10000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
