Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, mouse heart tissue, HepG2 cells, Jurkat cells, mouse brain, rat brain, rat heart tissue |
| Positive IP detected in | HepG2 cells, mouse heart tissue |
| Positive IHC detected in | human bowen disease tissue, human kidney tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21632-1-AP targets KIF5B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16250 Product name: Recombinant human KIF5B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 662-782 aa of BC126279 Sequence: ESVDALSEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKAKLITDLQDQNQKMMLEQERLRVEHEKLKATDQEKSRKLHELTVMQDRREQARQDL Predict reactive species |
| Full Name | kinesin family member 5B |
| Calculated Molecular Weight | 963 aa, 110 kDa |
| Observed Molecular Weight | 110-120 kDa |
| GenBank Accession Number | BC126279 |
| Gene Symbol | KIF5B |
| Gene ID (NCBI) | 3799 |
| RRID | AB_11182931 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P33176 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KIF5B, also known as KINH, KNS1, belongs to the Kinesin-1 family. KIF5B contains three structural domains: a globular N-terminal motor domain, a central alpha-helical rod domain, and a globular C-terminal domain. During the G2 phase of the cell cycle in a BICD2-dependent manner, KIF5B antagonizes the dynein function and drives the separation of nuclei and centrosomes (PMID: 20386726). KIF5B drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (KIF5B:24088571).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KIF5B antibody 21632-1-AP | Download protocol |
| IHC protocol for KIF5B antibody 21632-1-AP | Download protocol |
| IP protocol for KIF5B antibody 21632-1-AP | Download protocol |
| WB protocol for KIF5B antibody 21632-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KIF5B Polyclonal Antibody (Cat# 21632-1-AP) has 40 citations across journals including Cell Metabolism, Science Advances, Cell Reports, Cell Death & Differentiation, Journal of Cell Biology, EMBO Reports, Neurobiology of Disease, Advanced Science, and eLife. Associated research fields encompass neuroscience (axonal transport, Alzheimer's disease, traumatic brain injury), mitochondrial biology, cancer biology, cytoskeletal dynamics, immunology, and virology.
| Species | Application | Title |
|---|---|---|
Cell Metab Excessive vigorous exercise impairs cognitive function through a muscle-derived mitochondrial pretender. | ||
Cell Metab Compartmentalized branched-chain amino acid metabolism orchestrates colorectal cancer dissemination via an UMP-vimentin axis. | ||
Bioact Mater Advanced nerve regeneration enabled by neural conformal electronic stimulators enhancing mitochondrial transport | ||
Exp Mol Med Activation of TLR7-mediated autophagy increases epileptic susceptibility via reduced KIF5A-dependent GABAA receptor transport in a murine model | ||
J Nanobiotechnology Static magnetic field-modulated mesenchymal stem cell-derived mitochondria-containing microvesicles for enhanced intervertebral disc degeneration therapy
| ||
Adv Sci (Weinh) ATL2 Recruits TRAK1 to Promote Mitochondrial Transport at ER-Mitochondria Contact Sites. |
Reviews
What customers say
All three reviews rate the antibody 5 stars. Users report excellent performance in Western blot and immunoprecipitation across multiple cell types and tissues, including SH-SY5Y cells, mouse brain, PC12, HEK293T, and endothelial cells. Dilutions of 1:1000–1:4000 for WB and 1–5 µg/mg for IP were used. Reviewers highlight a single clean band at the expected molecular weight, strong signal, and sufficient antibody concentration for multiple experiments. One user noted that room-temperature incubation for 2 hours worked perfectly. Overall, the antibody is described as reliable, high-titer, and well-suited for both detection and pull-down applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Susana (Verified Customer) (11-30-2022) | The antibody works very well on western blotting. I did an incubation at room temperature for 2 hours and it worked perfectly; I had one band at the expected molecular weight.
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Xu (Verified Customer) (10-06-2020) | This one contains the image. I specifically probes KIF5B from both mouse brain and PC12/HEK293T cells. It can also be used in IP with a high titer. Awesome!
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D (Verified Customer) (09-26-2019) | Works excellent in western blots and immunoprecipitations. Good antibody concentration for doing multiple experiments.
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