Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse brain, mouse heart, rat brain |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human kidney tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 24 publications below |
| IHC | See 1 publications below |
| IF | See 7 publications below |
| IP | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
21632-1-AP targets KIF5B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16250 Product name: Recombinant human KIF5B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 662-782 aa of BC126279 Sequence: ESVDALSEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLRDEVEAKAKLITDLQDQNQKMMLEQERLRVEHEKLKATDQEKSRKLHELTVMQDRREQARQDL Predict reactive species |
| Full Name | kinesin family member 5B |
| Calculated Molecular Weight | 963 aa, 110 kDa |
| Observed Molecular Weight | 110-120 kDa |
| GenBank Accession Number | BC126279 |
| Gene Symbol | KIF5B |
| Gene ID (NCBI) | 3799 |
| RRID | AB_11182931 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P33176 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KIF5B, also known as KINH, KNS1, belongs to the Kinesin-1 family. KIF5B contains three structural domains: a globular N-terminal motor domain, a central alpha-helical rod domain, and a globular C-terminal domain. During the G2 phase of the cell cycle in a BICD2-dependent manner, KIF5B antagonizes the dynein function and drives the separation of nuclei and centrosomes (PMID: 20386726). KIF5B drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (KIF5B:24088571).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KIF5B antibody 21632-1-AP | Download protocol |
| IHC protocol for KIF5B antibody 21632-1-AP | Download protocol |
| IP protocol for KIF5B antibody 21632-1-AP | Download protocol |
| WB protocol for KIF5B antibody 21632-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Arterioscler Thromb Vasc Biol Kinesin-1: A New Actor Involved in Platelet Secretion and Thrombus Stability.
| ||
J Hazard Mater Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria. | ||
J Cell Biol Kinesin-1 controls mast cell degranulation and anaphylaxis through PI3K-dependent recruitment to the granular Slp3/Rab27b complex.
| ||
EMBO Rep MICAL2PV suppresses the formation of tunneling nanotubes and modulates mitochondrial trafficking. | ||
Cancers (Basel) GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Susana (Verified Customer) (11-30-2022) | The antibody works very well on western blotting. I did an incubation at room temperature for 2 hours and it worked perfectly; I had one band at the expected molecular weight.
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FH Xu (Verified Customer) (10-06-2020) | This one contains the image. I specifically probes KIF5B from both mouse brain and PC12/HEK293T cells. It can also be used in IP with a high titer. Awesome!
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FH D (Verified Customer) (09-26-2019) | Works excellent in western blots and immunoprecipitations. Good antibody concentration for doing multiple experiments.
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