KIF5B Recombinant antibody

• Featured Product
• KD/KO Validated

KIF5B Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B18

Cat no : 82485-1-RR

Synonyms

KIF5B, Kinesin 1 heavy chain, kinesin family member 5B, KINH, KNS, KNS1, Ubiquitous kinesin heavy chain, UKHC

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Validation Data Gallery

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  WB analysis of HeLa using 82485-1-RR

  WB result of KIF5B antibody (82485-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF5B transfected HeLa cells.

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  WB analysis using 82485-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82485-1-RR (KIF5B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, A549 cells, Jurkat cells, mouse heart tissue, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82485-1-RR targets KIF5B in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KIF5B fusion protein Ag16250
• Full Name: kinesin family member 5B
• Calculated Molecular Weight: 963 aa, 110 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC126279
• Gene Symbol: KIF5B
• Gene ID (NCBI): 3799
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIF5B, also known as KINH, KNS1, belongs to the Kinesin-1 family. KIF5B contains three structural domains: a globular N-terminal motor domain, a central alpha-helical rod domain, and a globular C-terminal domain. During the G2 phase of the cell cycle in a BICD2-dependent manner, KIF5B antagonizes the dynein function and drives the separation of nuclei and centrosomes (PMID: 20386726). KIF5B drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (KIF5B:24088571).