Tested Applications
| Positive WB detected in | human brain tissue, mouse testis tissue, mouse brain tissue, MCF-7 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12700-1-AP targets KIFAP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3394 Product name: Recombinant human KIFAP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 491-792 aa of BC028679 Sequence: SQHDGPTKNLFIDYVGDLAAQISNDEEEEFVIECLGTLANLTIPDLDWELVLKEYKLVPYLKDKLKPGAAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVESRQMDESEQYLYGDDRIEPYIHEGDILERPDLFYNSDGLIASEGAISPDFFNDYHLQNGDVVGQHSFPGSLGMDGFGQPVGILGRPATAYGFRPDEPYYYGYGS Predict reactive species |
| Full Name | kinesin-associated protein 3 |
| Calculated Molecular Weight | 792 aa, 91 kDa |
| Observed Molecular Weight | 91-100 kDa |
| GenBank Accession Number | BC028679 |
| Gene Symbol | KIFAP3 |
| Gene ID (NCBI) | 22920 |
| RRID | AB_2132528 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92845 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KIFAP3, also known as KIF3AP or KAP3, is a novel KIF3A/3B-associated protein. It binds to the tail domain of KIF3A/3B and may play a role in regulating the binding of KIF3A/3B to cargoes. Recently it has been reported that mutation within the KIFAP3 gene is associated with decreased KIFAP3 expression and increased survival in sporadic ALS, which makes it a potential target for ALS therapy. KIFAP3 has also been identified as the target of mir-130a.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KIFAP3 antibody 12700-1-AP | Download protocol |
| IHC protocol for KIFAP3 antibody 12700-1-AP | Download protocol |
| IP protocol for KIFAP3 antibody 12700-1-AP | Download protocol |
| WB protocol for KIFAP3 antibody 12700-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KIFAP3 Polyclonal antibody (Cat# 12700-1-AP) has 2 total citations. These appear in Nucleic Acids Research (2012), where it was used in a functional assay for microRNA target identification and validation, and in Communications Biology (2023), where it was applied to study the role of germline factor DDX4 in chemoresistance of small cell lung cancer cells. Both citations used Western blot on human samples. The research fields span RNA biology/microRNA biology and oncology.
| Species | Application | Title |
|---|---|---|
Commun Biol The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells |
