KIFC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

KIFC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA, Peptide array

Conjugate

Unconjugated

Publications

11

Cat no : 20790-1-AP

Synonyms

KNSL2, Kinesin-related protein HSET, Kinesin-like protein KIFC1, Kinesin-like protein 2, HSET

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Validation Data Gallery

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  WB analysis of rat testis using 20790-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 20790-1-AP (KIFC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 20790-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20790-1-AP (KIFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20790-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20790-1-AP (KIFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 20790-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20790-1-AP (KIFC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human bowen disease using 20790-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 20790-1-AP (KIFC1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 20790-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 20790-1-AP (KIFC1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20790-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20790-1-AP (KIFC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20790-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20790-1-AP (KIFC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 20790-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20790-1-AP (KIFC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 20790-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20790-1-AP (KIFC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 20790-1-AP

  Immunofluorescent analysis of Hela cells, using KIFC1 antibody 20790-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HepG2 using 20790-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIFC1 antibody 20790-1-AP at 1:25 dilution and FITC- labeled donkey anti-Rabbit IgG (green).

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Tested Applications

• Positive WB detected in: rat testis tissue, HeLa cells, mouse testis tissue, NIH/3T3 cells
• Positive IHC detected in: human bowen disease, human placenta tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

20790-1-AP targets KIFC1 in WB, IHC, IF/ICC, ELISA, Peptide array applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIFC1 fusion protein Ag14492
• Full Name: kinesin family member C1
• Calculated Molecular Weight: 673 aa, 74 kDa
• Observed Molecular Weight: 55-70 kDa
• GenBank Accession Number: BC000712
• Gene Symbol: KIFC1
• Gene ID (NCBI): 3833
• RRID: AB_10755286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIFC1 is a molecular motor protein belonging to the kinesin-14 family that has motor domains at the C-terminus. KIFC1 is widely distributed, with highest expression in testis and participating in acrosome biogenesis and vesicle transport. It is also abundant in ovary, spleen, and liver. KIFC1 proteins are generally localized in the perinuclear region, and have been found to be involved chromosome alignment, congression and intracellular DNA transport. This antibody was raised against the C-terminal region of human KIFC1 protein, and recognizes 55-70 kDa of endogenous KIFC1 protein in multiple tissue lysates. (12826589, 15236353, 23543461)

Publications

Species	Application	Title
mouse	WB,IF,Peptide array	Science; Mechanism of spindle pole organization and instability in human oocytes.; Authors - Chun So ; KD Validated
human	WB	Dev Cell; Direct Microtubule-Binding by Myosin-10 Orients Centrosomes toward Retraction Fibers and Subcortical Actin Clouds.; Authors - Mijung Kwon
human	WB	Cell Mol Life Sci; Proteomic interaction profiling reveals KIFC1 as a factor involved in early targeting of F508del-CFTR to degradation.; Authors - Sara Canato ; KD Validated
rat	WB	J Neurosci; KIFC1 regulates the trajectory of neuronal migration.; Authors - Hemalatha Muralidharan ; KD Validated
human	IF	FASEB J; Export of membrane proteins from the Golgi complex to the primary cilium requires the kinesin motor, KIFC1.; Authors - Si-Hyung Lee
human	IHC	Front Mol Biosci; Prognostic Value and Immunological Role of KIFC1 in Hepatocellular Carcinoma.; Authors - Dan Li