KIFC3 Polyclonal antibody

• Featured Product
• KD/KO Validated

KIFC3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 10125-2-AP

Synonyms

Kinesin-like protein KIFC3

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Validation Data Gallery

• 

  WB analysis of mouse lung using 10125-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10125-2-AP (KIFC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10125-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10125-2-AP (KIFC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 10125-2-AP

  IP result of anti-KIFC3 (IP:10125-2-AP, 4ug; Detection:10125-2-AP 1:500) with mouse kidney tissue lysate 7200ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 10125-2-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10125-2-AP (KIFC3 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse kidney tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

10125-2-AP targets KIFC3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIFC3 fusion protein Ag0172
• Full Name: kinesin family member C3
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 80-85 kDa, 95-100 kDa
• GenBank Accession Number: BC001211
• Gene Symbol: KIFC3
• Gene ID (NCBI): 3801
• RRID: AB_2296476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Transiently formed nucleus-to-cilium microtubule arrays mediate senescence initiation in a KIFC3-dependent manner; Authors - Jielu Hao Robichaud
human	WB	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated
human	WB	Cancer Res; PMID: 19789344; Authors - De Sarmishtha S
mouse,rat	WB	Mol Biol Cell; Kif5B and Kifc1 interact and are required for motility and fission of early endocytic vesicles in mouse liver.; Authors - Nath Sangeeta S
human	WB	World J Gastrointest Oncol; KIFC3 promotes proliferation, migration and invasion of esophageal squamous cell carcinoma cells by activating EMT and β-catenin signaling; Authors - Wei-Wei Hao ; KD Validated
human	WB	Biochim Biophys Acta; The iron-regulated metastasis suppressor, Ndrg-1: identification of novel molecular targets.; Authors - Kovacevic Zaklina Z