KIR2DL1 Recombinant monoclonal antibody

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  WB analysis using 85168-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85168-1-RR (KIR2DL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85168-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85168-1-RR against Human KIR2DL1 were performed. The affinity constant is 37.9 nM.

KIR2DL1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85168-1-RR

Clone No.242070D11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

242070D11, CD158 antigen-like family member A, CD158a, Killer cell immunoglobulin-like receptor 2DL1, Natural killer-associated transcript 1

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85168-1-RR targets KIR2DL1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC069344
• Gene Symbol: KIR2DL1
• Gene ID (NCBI): 3802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P43626
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Killer cell immunoglobulin-like receptor 2DL1 (KIR2DL1, also known as CD158A and NKAT1) is an inhibitory receptor of NK cells, with β-arrestin 2 mediated recruitment of the tyrosine phosphatases SHP-1 and SHP-2 to KIR2DL1 and facilitated downstream inhibitory signaling (PMID: 18604210). The effector function of NK cells expressing KIR2DL1 or KIR2DS1 is highly influenced by genetic variability and is calibrated by co-expression of additional NK receptors and cognate HLA-C2 ligands (PMID: 31024561).