KIR3DL1 Polyclonal antibody

KIR3DL1 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC,ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13335-1-AP

Synonyms

CD158E, CD158E1, KIR, KIR3DL1, MHC class I NK cell receptor, NKAT 3, NKAT3, NKB1, NKB1B, p70 NK receptor CL 2/CL 11

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Validation Data Gallery

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  WB analysis of human liver using 13335-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13335-1-AP (KIR3DL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 13335-1-AP

  1X10^6 Jurkat cells were stained with 0.2ug KIR3DL1 antibody (13335-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: human liver tissue
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13335-1-AP targets KIR3DL1 in WB, FC,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIR3DL1 fusion protein Ag4152
• Full Name: killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1
• Calculated Molecular Weight: 444 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC028206
• Gene Symbol: KIR3DL1
• Gene ID (NCBI): 3811
• RRID: AB_2234189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR3DL1 is a receptor on natural killer (NK) cells for HLA Bw4 allele. It inhibits the activity of NK cells thus preventing cell lysis. KIR3DL1 and KIR3DS1 are highly homologous. This antibody is raised against 22-344 amino acids of human KIR3DL1 and recognizes both KIR3DL1 (49 kDa) and KIR3DS1 (43 kDa).

Publications

Species	Application	Title
		Clin Exp Immunol; Autoantibodies to Killer Cell Immunoglobulin-Like Receptor 3DL1 in Patients with Systemic Lupus Erythematosus.; Authors - Akihiro Doi