KISS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

KISS1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 18375-1-AP

Synonyms

KiSS 1, KiSS 1 metastasis suppressor, KISS1, Kisspeptin 1, Metastasis suppressor KiSS 1, metastin

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 18375-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18375-1-AP (KISS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 18375-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18375-1-AP (KISS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 18375-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18375-1-AP (KISS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 18375-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18375-1-AP (KISS1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 18375-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18375-1-AP (KISS1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 18375-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18375-1-AP (KISS1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of NIH/3T3 using 18375-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 18375-1-AP (KISS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, human placenta tissue, mouse brain tissue
• Positive IHC detected in: human liver cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below

Product Information

The immunogen of 18375-1-AP is KISS1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KISS1 fusion protein Ag13240
• Full Name: KiSS-1 metastasis-suppressor
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 16-20 kDa
• GenBank Accession Number: BC022819
• Gene Symbol: KISS1
• Gene ID (NCBI): 3814
• RRID: AB_11182705
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KISS1, also known as kisspeptin, is a metastasis suppressor protein that suppresses metastases of human melanomas and breast carcinomas without affecting tumorigenicity. It may regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A C-terminally amidated peptide of KISS1, metastin (kisspeptin-54 or kp-54), functions as the endogenous ligand of the G-protein coupled receptor GPR54 (PMID: 11457843; 11385580). KISS1-GPR54 signaling is involved in the regulation of reproduction, playing an important role in initiating secretion of gonadotropin-releasing hormone (GnRH) at puberty (PMID: 18753370).

Publications

Species	Application	Title
human	WB,IHC	Int J Cancer; Long noncoding RNA TC0101441 induces epithelial-mesenchymal transition in epithelial ovarian cancer metastasis by downregulating KiSS1.; Authors - Jun-Jun Qiu ; KD Validated
human	IHC	Histochem Cell Biol; Functional analysis of miR-767-5p during the progression of hepatocellular carcinoma and the clinical relevance of its dysregulation.; Authors - Lei Zhang
human	IHC	Oncol Lett; Analysis of the association of the expression of KiSS-1 in colorectal cancer tissues with the pathology and prognosis.; Authors - Xinkai Huo
human	WB	Mol Oncol; Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis; Authors - Tianwei Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kristina (Verified Customer) (09-18-2020)

Works great for us- seeing an increase in modified MDA231 cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MDA-MB-231 with modifications