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KISS1 Polyclonal antibody

KISS1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18375-1-AP

Synonyms

KiSS 1, KiSS 1 metastasis suppressor, KISS1, Kisspeptin 1, Metastasis suppressor KiSS 1, metastin



Tested Applications

Positive WB detected inNIH/3T3 cells, human placenta tissue, mouse brain tissue
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18375-1-AP is KISS1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KISS1 fusion protein Ag13240
Full Name KiSS-1 metastasis-suppressor
Calculated molecular weight 15 kDa
Observed molecular weight 16-20 kDa
GenBank accession numberBC022819
Gene symbol KISS1
Gene ID (NCBI) 3814
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KISS1, also known as kisspeptin, is a metastasis suppressor protein that suppresses metastases of human melanomas and breast carcinomas without affecting tumorigenicity. It may regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A C-terminally amidated peptide of KISS1, metastin (kisspeptin-54 or kp-54), functions as the endogenous ligand of the G-protein coupled receptor GPR54 (PMID: 11457843; 11385580). KISS1-GPR54 signaling is involved in the regulation of reproduction, playing an important role in initiating secretion of gonadotropin-releasing hormone (GnRH) at puberty (PMID: 18753370).

Protocols

Product Specific Protocols
WB protocol for KISS1 antibody 18375-1-APDownload protocol
IHC protocol for KISS1 antibody 18375-1-APDownload protocol
IF protocol for KISS1 antibody 18375-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncol Lett

Analysis of the association of the expression of KiSS-1 in colorectal cancer tissues with the pathology and prognosis.

Authors - Xinkai Huo
humanIHC

Histochem Cell Biol

Functional analysis of miR-767-5p during the progression of hepatocellular carcinoma and the clinical relevance of its dysregulation.

Authors - Lei Zhang
humanWB,IHC

Int J Cancer

Long noncoding RNA TC0101441 induces epithelial-mesenchymal transition in epithelial ovarian cancer metastasis by downregulating KiSS1.

Authors - Jun-Jun Qiu
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kristina (Verified Customer) (09-18-2020)

Works great for us- seeing an increase in modified MDA231 cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: MDA-MB-231 with modifications