KLF10 Polyclonal antibody

KLF10 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF, IHC, Chip, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11881-1-AP

Synonyms

EGR alpha, EGRA, KLF10, Krueppel like factor 10, Kruppel like factor 10, TIEG, TIEG 1, TIEG1

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Validation Data Gallery

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  WB analysis of HeLa using 11881-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11881-1-AP (KLF10 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11881-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11881-1-AP (KLF10 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 11881-1-AP

  IP result of anti-KLF10 (IP:11881-1-AP, 4ug; Detection:11881-1-AP 1:300) with Jurkat cells lysate 2800ug.

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  IF Staining of HeLa using 11881-1-AP

  Immunofluorescent analysis of HeLa cells using 11881-1-AP (KLF10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Product Information

11881-1-AP targets KLF10 in WB, IP, IF, IHC, Chip, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLF10 fusion protein Ag2482
• Full Name: Kruppel-like factor 10
• Calculated Molecular Weight: 469 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC011538
• Gene Symbol: KLF10
• Gene ID (NCBI): 7071
• RRID: AB_2877801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLF10 (Krueppel-like factor 10), also known as TIEG1, is a transcription factor that plays a role in the regulation of cell growth. It binds to the consensus sequence 5'-GGTGTG-3'. KLF10 plays a role in the response to TGFbeta (through the TGFbeta/Smad signalling pathway) and estrogen. In both cases, KLF10 induces or repressed gene transcription to enhance the TGFbeta/Smad signalling pathway, as well as other pathways, to regulate cell proliferation, differentiation and apoptosis. KLF10 has been shown to have a role in skeletal disease (osteopenia/osteoporosis), hypertrophic cardiomyopathy, and breast and prostate cancer.This antibody is a rabbit polyclonal antibody raised against a full-length human KLF10 protein, and specifically recognizes the 52kd KLF10 protein.

Publications

Species	Application	Title
human	Chip	Sci Rep; Polymorphism rs7278468 is associated with Age-related cataract through decreasing transcriptional activity of the CRYAA promoter.; Authors - Xiaoyin Ma
human	IF	J Mol Cell Cardiol; Cellular heterogeneity and immune microenvironment revealed by single-cell transcriptome in venous malformation and cavernous venous malformation.; Authors - Yongyun Li
human	WB,IHC	Acta Histochem; KLF10 is upregulated in osteoarthritis and inhibits chondrocyte proliferation and migration by upregulating Acvr1 and suppressing inhbb expression.; Authors - Langlang Zheng
mouse,canine	IF	Cell Death Discov; Downregulation of KLF10 contributes to the regeneration of survived renal tubular cells in cisplatin-induced acute kidney injury via ZBTB7A-KLF10-PTEN axis; Authors - Yang Zhang
mouse,human	WB	Cell Signal; Mammalian cleavage factor 25 targets KLF14 to inhibit hepatic stellate cell activation and liver fibrosis; Authors - Xiaoyan Chen