Tested Applications
| Positive WB detected in | mouse embryo tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| ChIP | See 1 publications below |
Product Information
34052-1-AP targets KLF11 in WB, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag40584 Product name: Recombinant human KLF11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC063286 Sequence: MHTPDFAGPDDARAVDIMDICESILERKRHDSERSTCSILEQTDMEAVEALVCMSSWGQRSQKGDLLRIRPLTPVSDSGDVTTTVHMDAATPELPKDFHSLSTLCITPPQSPDLVEPSTRTPVSPQVTDSKACTATDVLQSSAVVARALSGGAERGLLGLEPVPSSPCRAKGTSVIRHTGESPAACFPTIQTPDCRLSDS Predict reactive species |
| Full Name | Kruppel-like factor 11 |
| Calculated Molecular Weight | 55 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC063286 |
| Gene Symbol | KLF11 |
| Gene ID (NCBI) | 8462 |
| RRID | AB_3743144 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O14901 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KLF11 (Krüppel-like factor 11) belongs to the zinc finger transcription factor family. Its C-terminus contains three tandem C2H2-type zinc finger domains responsible for sequence-specific DNA binding. It regulates transcription by binding to the GC box or CACCC element of target gene promoters, and in the pancreas, it negatively regulates insulin gene expression as a transcriptional repressor. Furthermore, KLF11 participates in cell cycle regulation, apoptosis, and fibrosis pathways, possesses tumor-suppressive functions.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KLF11 antibody 34052-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
