Tested Applications
| Positive WB detected in | HT-29 cells, rat liver tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18352-1-AP targets KLF13 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13200 Product name: Recombinant human KLF13 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-288 aa of BC012741 Sequence: MAAAAYVDHFAAECLVSMSSRAVVHGPREGPESRPEGAAVAATPTLPRVEERRDGKDSASLFVVARILADLNQQAPAPAPAERREGAAARKARTPCRLPPPAPEPTSPGAEGAAAAPPSPAWSEPEPEAGLEPEREPGPAGSGEPGLRQRVRRGRSRADLESPQRKHKCHYAGCEKVYGKSSHLKAHLRTHTGERPFACSWQDCNKKFARSDELARHYRTHTGEKKFSCPICEKRFMRSDHLTKHARRHANFHPGMLQRRGGGSRTGSLSDYSRSDASSPTISPASSP Predict reactive species |
| Full Name | Kruppel-like factor 13 |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 37-45 kDa |
| GenBank Accession Number | BC012741 |
| Gene Symbol | KLF13 |
| Gene ID (NCBI) | 51621 |
| RRID | AB_2132399 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2Y9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Kruppel-like factor 13(KLF13) is a member of transcription actors, which contains 3 zinc finger DNA-binding domains. Theses transcription factors bind to CG-rich sequences and related GT and CACCC boxes. KLF13 involved in activation of transcription of variety of genes in the cells of erythroid lineage, such as GATA1, glycophorin B. It also binds to the A and A/B RANTES promoter binding sites, the same as NFKB, and then transactivates the RANTES gene. The calculated molecular weight of KLF13 is 31 kDa, but the modified KLF13 protein is about 37-45 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KLF13 antibody 18352-1-AP | Download protocol |
| WB protocol for KLF13 antibody 18352-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KLF13 antibody (Cat# 18352-1-AP) has 15 citations published in journals including Nature, iScience, J Biol Chem, Cell Biosci, J Clin Transl Hepatol, Biol Direct, Chem Biol Interact, J Cell Physiol, J Hepatocell Carcinoma, J Microbiol, Placenta, Int J Exp Pathol, Discov Oncol, Hum Cell, and Onco Targets Ther. Research fields span oncology (colorectal, liver, gastric, thyroid, and glioma cancers), cardiology, cholesterol metabolism, neurology, preeclampsia, and sepsis-related inflammation.
| Species | Application | Title |
|---|---|---|
Cell Biosci KLF13 suppresses the proliferation and growth of colorectal cancer cells through transcriptionally inhibiting HMGCS1-mediated cholesterol biosynthesis.
| ||
J Clin Transl Hepatol Krüppel-like Factor 13 Promotes HCC Progression by Transcriptional Regulation of HMGCS1-mediated Cholesterol Synthesis
| ||
Biol Direct Kruppel-like factor 13 acts as a tumor suppressor in thyroid carcinoma by downregulating IFIT1 | ||
Chem Biol Interact Gypenosides suppress hepatocellular carcinoma cells by blocking cholesterol biosynthesis through inhibition of MVA pathway enzyme HMGCS1 | ||
iScience KLF13-mediated CES2 upregulation via p300-dependent acetylation sensitizes gastric cancer cells to irinotecan.
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