KLF5 Recombinant monoclonal antibody

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  IF Staining of HeLa using 83205-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KLF5 antibody (83205-4-RR, Clone: 230509G7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of A549 using 83205-4-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Klf5 Recombinant Antibody (83205-4-RR, Clone:230509G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of HT-29 using 83205-4-RR

  1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Klf5 Recombinant Antibody (83205-4-RR, Clone:230509G7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83205-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83205-4-RR against Human KLF5 were performed. The affinity constant is 2.36 nM.

KLF5 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Cat No. 83205-4-RR

Clone No.230509G7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: A549 cells, HT-29 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83205-4-RR targets KLF5 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag15267

  Product name: Recombinant human KLF5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC042131

  Sequence: MATRVLSMSARLGPVPQPPAPQDEPVFAQLKPVLGAANPARDAALFPGEELKHAHHRPQAQPAPAQAPQPAQPPATGPRLPPEDLVQTRCEMEKYLTPQLPPVPIIPEHKKYRRDSASVVDQFFTDTEGLPYSINMNVFLPDITHLRTGLYKSQRPCVTHIKTEPVAIFSHQSETTAPPPAPTQALPEFTSIFSSHQTAAPEVNNIFIKQELPTPDLHLSVPTQQGHLYQLLNTPDLDMPSSTNQTAAMDTLNVSMSAAMAGLNTHTSAVPQTAVKQFQGMPPCTYTMPSQFLPQQATYFPPSPPSSEPGSPDRQAEMLQNLTPPPSYAATIASKLAIHNPNLPTTLPVN

• Full Name: Kruppel-like factor 5 (intestinal)
• Calculated Molecular Weight: 457 aa, 51 kDa
• Observed Molecular Weight: 51-57 kDa
• GenBank Accession Number: BC042131
• Gene Symbol: KLF5
• Gene ID (NCBI): 688
• RRID: AB_3670893
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q13887
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling.