KLHDC5 Polyclonal antibody

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• KLHDC5 Polyclonal antibody (24847-1-AP)

KLHDC5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24847-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ctb9, Cullin-3-binding protein 9, kelch domain containing 5, Kelch domain-containing protein 5, Kelch-like protein 42

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  WB analysis of HeLa using 24847-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24847-1-AP (KLHDC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 24847-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24847-1-AP (KLHDC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 24847-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24847-1-AP (KLHDC5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 24847-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24847-1-AP (KLHDC5 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 24847-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24847-1-AP (KLHDC5 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 24847-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24847-1-AP (KLHDC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 24847-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24847-1-AP (KLHDC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 24847-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24847-1-AP (KLHDC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse cerebellum using 24847-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24847-1-AP (KLHDC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse pancreas using 24847-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 24847-1-AP (KLHDC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 24847-1-AP

  Immunofluorescent analysis of HeLa cells using 24847-1-AP (KLHDC5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, HepG2 cells, PC-3 cells, mouse thymus tissue, mouse uterus tissue
• Positive IHC detected in: human kidney tissue, human pancreas cancer tissue, mouse kidney tissue, human placenta tissue, mouse cerebellum tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24847-1-AP targets KLHDC5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20448

  Product name: Recombinant human KLHDC5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 157-505 aa of BC137116

  Sequence: FHEVLCKPQFHLLGSPPQAPGDVSLKQRLREARMTGTPVLVALGDFLGGPLAPHPYQGEPPSMLRYEEMTERWFPLANNLPPDLVNVRGYGSAILDNYLFIVGGYRITSQEISAAHSYNPSTNEWLQVASMNQKRSNFKLVAVNSKLYAIGGQAVSNVECYNPEQDAWNFVAPLPNPLAEFSACECKGKIYVIGGYTTRDRNMNILQYCPSSDMWTLFETCDVHIRKQQMVSVEETIYIVGGCLHELGPNRRSSQSEDMLTVQSYNTVTRQWLYLKENTSKSGLNLTCALHNDGIYIMSRDVTLSTSLEHRVFLKYNIFSDSWEAFRRFPAFGHNLLVSSLYLPNKAET

• Full Name: kelch domain containing 5
• Calculated Molecular Weight: 505 aa, 57 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC137116
• Gene Symbol: KLHDC5
• Gene ID (NCBI): 57542
• RRID: AB_2879754
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P2K6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLHDC5 also named as kelch domain containing 5 or KLHL42 is a 505 amino acid protein, which contains one BTB domain and six Kelch repeats. KLHDC5 localizes in cytoplasm and may involve in microtubule dynamics throughout mitosis.

Publications

What published studies show

The KLHDC5 Polyclonal antibody (Cat# 24847-1-AP) has 1 total citation. It appears in Acta Pharmacologica Sinica (IF 10.4), published in 2023. The cited study investigated satellite cell-derived exosome-mediated delivery of a microRNA cluster to ameliorate renal tubulointerstitial fibrosis in mouse diabetic nephropathy, placing it in the fields of nephrology and diabetic kidney disease research. The antibody was applied via WB and IHC in both mouse and human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IHC	Acta Pharmacol Sin; Satellite cell-derived exosome-mediated delivery of microRNA-23a/27a/26a cluster ameliorates the renal tubulointerstitial fibrosis in mouse diabetic nephropathy; Authors - Jia-Ling Ji