KLHDC5 Polyclonal antibody

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  WB analysis of mouse brain using 24847-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24847-1-AP (KLHDC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24847-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24847-1-AP (KLHDC5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 24847-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24847-1-AP (KLHDC5 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 24847-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24847-1-AP (KLHDC5 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 24847-1-AP

  Immunofluorescent analysis of HeLa cells using 24847-1-AP (KLHDC5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

KLHDC5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24847-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ctb9, Cullin-3-binding protein 9, kelch domain containing 5, Kelch domain-containing protein 5, Kelch-like protein 42

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

24847-1-AP targets KLHDC5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20448

  Product name: Recombinant human KLHDC5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 157-505 aa of BC137116

  Sequence: FHEVLCKPQFHLLGSPPQAPGDVSLKQRLREARMTGTPVLVALGDFLGGPLAPHPYQGEPPSMLRYEEMTERWFPLANNLPPDLVNVRGYGSAILDNYLFIVGGYRITSQEISAAHSYNPSTNEWLQVASMNQKRSNFKLVAVNSKLYAIGGQAVSNVECYNPEQDAWNFVAPLPNPLAEFSACECKGKIYVIGGYTTRDRNMNILQYCPSSDMWTLFETCDVHIRKQQMVSVEETIYIVGGCLHELGPNRRSSQSEDMLTVQSYNTVTRQWLYLKENTSKSGLNLTCALHNDGIYIMSRDVTLSTSLEHRVFLKYNIFSDSWEAFRRFPAFGHNLLVSSLYLPNKAET

• Full Name: kelch domain containing 5
• Calculated Molecular Weight: 505 aa, 57 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC137116
• Gene Symbol: KLHDC5
• Gene ID (NCBI): 57542
• RRID: AB_2879754
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P2K6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLHDC5 also named as kelch domain containing 5 or KLHL42 is a 505 amino acid protein, which contains one BTB domain and six Kelch repeats. KLHDC5 localizes in cytoplasm and may involve in microtubule dynamics throughout mitosis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IHC	Acta Pharmacol Sin; Satellite cell-derived exosome-mediated delivery of microRNA-23a/27a/26a cluster ameliorates the renal tubulointerstitial fibrosis in mouse diabetic nephropathy; Authors - Jia-Ling Ji