KLHL14 Polyclonal antibody

KLHL14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51131-1-AP

Synonyms

kelch like 14 (Drosophila), Kelch like protein 14, KIAA1384, KLHL14

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 51131-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 51131-1-AP (KLHL14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 51131-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51131-1-AP (KLHL14 antibody) at dilution of 1:50 (under 10x lens).

---

Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

51131-1-AP targets KLHL14 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: kelch-like 14 (Drosophila)
• Calculated Molecular Weight: 44 kDa, 71 kDa
• Observed Molecular Weight: 55 kDa, 70 kDa
• GenBank Accession Number: BC021267
• Gene Symbol: KLHL14
• Gene ID (NCBI): 57565
• RRID: AB_2131168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.